Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Erdemli, S.B.; Gupta, R.; Bishai, W.R.; Lamichhane, G.; Amzel, L.M.; Bianchet, M.A.
    Targeting the cell wall of Mycobacterium tuberculosis: structure and mechanism of L,D-transpeptidase 2 (2012), Structure, 20, 2103-2115.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.B14 the extramembrane portion of the L,D-transpeptidase is expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.B14 hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 1 M succinic acid, and 1% (w/v) PEG MME 2000 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.B14 C354A inactive Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.B14 ertapenem
-
Mycobacterium tuberculosis
2.3.2.B14 Imipenem irreversible inhibition, imipenem binds only the catalytically active enzyme with apparent submicromolar affinity Mycobacterium tuberculosis
2.3.2.B14 meropenem meropenem binds only the catalytically active enzyme with apparent submicromolar affinity Mycobacterium tuberculosis
2.3.2.B14 additional information not inhibited by clavulanic acid Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.B14 cell wall
-
Mycobacterium tuberculosis 5618
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.B14 Mycobacterium tuberculosis O53223
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.B14 nickel affinity column chromatography and Sepharose 12 column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.B14 additional information the enzyme also shows low beta-lactamase activity with nitrocefin (i.e. 3-(2,4-dinitrostyryl)-(6R,7R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxylic acid) with Km of 0.016 mM and kcat of 0.01 s-1 (in 20 mM HEPES (pH 7.5) and 300 mM NaCl, temperature not specified in the publication) Mycobacterium tuberculosis ?
-
?

Subunits

EC Number Subunits Comment Organism
2.3.2.B14 monomer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.2.B14 L,D-transpeptidase 2
-
Mycobacterium tuberculosis
2.3.2.B14 LdtMt2
-
Mycobacterium tuberculosis
2.3.2.B14 MT0125
-
Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.B14 7.8
-
pH optimum for beta-lactamase activity with nitrocefin Mycobacterium tuberculosis