Literature summary extracted from
Erdemli, S.B.; Gupta, R.; Bishai, W.R.; Lamichhane, G.; Amzel, L.M.; Bianchet, M.A.
Targeting the cell wall of Mycobacterium tuberculosis: structure and mechanism of L,D-transpeptidase 2 (2012), Structure, 20, 2103-2115.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.3.2.B14 |
the extramembrane portion of the L,D-transpeptidase is expressed in Escherichia coli BL21(DE3) cells |
Mycobacterium tuberculosis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.3.2.B14 |
hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 1 M succinic acid, and 1% (w/v) PEG MME 2000 |
Mycobacterium tuberculosis |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.3.2.B14 |
C354A |
inactive |
Mycobacterium tuberculosis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.3.2.B14 |
ertapenem |
- |
Mycobacterium tuberculosis |
|
2.3.2.B14 |
Imipenem |
irreversible inhibition, imipenem binds only the catalytically active enzyme with apparent submicromolar affinity |
Mycobacterium tuberculosis |
|
2.3.2.B14 |
meropenem |
meropenem binds only the catalytically active enzyme with apparent submicromolar affinity |
Mycobacterium tuberculosis |
|
2.3.2.B14 |
additional information |
not inhibited by clavulanic acid |
Mycobacterium tuberculosis |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
2.3.2.B14 |
cell wall |
- |
Mycobacterium tuberculosis |
5618 |
- |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.2.B14 |
Mycobacterium tuberculosis |
O53223 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.3.2.B14 |
nickel affinity column chromatography and Sepharose 12 column chromatography |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.2.B14 |
additional information |
the enzyme also shows low beta-lactamase activity with nitrocefin (i.e. 3-(2,4-dinitrostyryl)-(6R,7R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxylic acid) with Km of 0.016 mM and kcat of 0.01 s-1 (in 20 mM HEPES (pH 7.5) and 300 mM NaCl, temperature not specified in the publication) |
Mycobacterium tuberculosis |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.3.2.B14 |
monomer |
x-ray crystallography |
Mycobacterium tuberculosis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.3.2.B14 |
L,D-transpeptidase 2 |
- |
Mycobacterium tuberculosis |
2.3.2.B14 |
LdtMt2 |
- |
Mycobacterium tuberculosis |
2.3.2.B14 |
MT0125 |
- |
Mycobacterium tuberculosis |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.3.2.B14 |
7.8 |
- |
pH optimum for beta-lactamase activity with nitrocefin |
Mycobacterium tuberculosis |