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Literature summary extracted from

  • Zhang, J.; Ma, B.; DiMaio, F.; Douglas, N.R.; Joachimiak, L.A.; Baker, D.; Frydman, J.; Levitt, M.; Chiu, W.
    Cryo-EM structure of a group II chaperonin in the prehydrolysis ATP-bound state leading to lid closure (2011), Structure, 19, 633-639.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
5.6.1.7 D386A in order to study the Mm-cpn in the ATP bound state, a lidless Mm-cpn variant (D386A DELTAlid Mm-cpn) is chosen. The mutation of Asp386 to an alanine makes the chaperonin ATPase deficient. This mutant can still bind but cannot hydrolyze ATP. Cryo-EM raw images and two-dimensional class-averages show D386A DELTAlid Mm-cpn is open in the ATP bound state as represented by rectangular-shaped side view and ring-shaped top view with clear subunit boundaries. This appears similar to those images of the D386A Mm-cpn without the lid deletion in the ATP-bound state Methanococcus maripaludis

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.7 Methanococcus maripaludis
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.7 ATP + H2O + a folded polypeptide the structure of an archaeal group II chaperonin in its prehydrolysis ATP-bound state at subnanometer resolution using single particle cryo-electron microscopyis reported. Structural comparison of Mm-cpn in ATP-free, ATP-bound, and ATP-hydrolysis states reveals that ATP binding alone causes the chaperonin to close slightly with a 45° counterclockwise rotation of the apical domain. The subsequent ATP hydrolysis drives each subunit to rock toward the folding chamber and to close the lid completely Methanococcus maripaludis ADP + phosphate + an unfolded polypeptide
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Synonyms

EC Number Synonyms Comment Organism
5.6.1.7 group II chaperonin
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 Methanococcus maripaludis
5.6.1.7 Mm-cpn 16 subunit homo-oligomeric chaperonin from archaea Methanococcus maripaludis (Mm-cpn) is used Methanococcus maripaludis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.1.7 37
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 assay at Methanococcus maripaludis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.1.7 7.5
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 assay at Methanococcus maripaludis