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Literature summary extracted from

  • Crawford, J.M.; Thomas, P.M.; Scheerer, J.R.; Vagstad, A.L.; Kelleher, N.L.; Townsend, C.A.
    Deconstruction of iterative multidomain polyketide synthase function (2008), Science, 320, 243-246.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.221 gene pksA, cloning of various mono-, di-, and tridomains for combinatorial reconstitution experiments, expression of the either C- or N-terminally His6-tagged protein fragments in Escherichia coli Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.221 7 malonyl-CoA + hexanoyl-[acyl-carrier protein] Aspergillus parasiticus
-
 7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.221 Aspergillus parasiticus
-
-
-
2.3.1.221 Aspergillus parasiticus
-
 gene pksA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.221 recombinant C- or N-terminally His6-tagged mono-, di-, and tridomains protein fragments from Escherichia coli by metal affinity cromatography Aspergillus parasiticus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.221 7 malonyl-CoA + hexanoyl-[acyl-carrier protein] = 7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O a product template domain unites with the ketosynthase and thioesterase in this IPKS system to assemble precisely seven malonyl-derived building blocks to a hexanoyl starter unit and mediate a specific cyclization cascade. These mechanistic features are general for IPKS-catalyzed production of aromatic polyketides Aspergillus parasiticus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.221 7 malonyl-CoA + hexanoyl-[acyl-carrier protein]
-
 Aspergillus parasiticus 7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.221 More domain structure, and dissection of domains, interactions, overview Aspergillus parasiticus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.221 norsolorinic acid anthrone synthase
-
 Aspergillus parasiticus
2.3.1.221 PksA
-
 Aspergillus parasiticus
2.3.1.221 polyketide synthase A
-
 Aspergillus parasiticus

General Information

EC Number General Information Comment Organism
2.3.1.221 metabolism PksA is involved in the synthesis of norsolorinic acid, an intermediate in the biosynthesis of the environmental carcinogen aflatoxin B1 Aspergillus parasiticus
2.3.1.221 physiological function PksA, which initiates biosynthesis of the environmental carcinogen aflatoxin B1, is one of the multidomain iterative polyketide synthases, IPKSs Aspergillus parasiticus