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Literature summary extracted from

  • Ragone, F.L.; Spears, J.L.; Wohlgamuth-Benedum, J.M.; Kreel, N.; Papavasiliou, F.N.; Alfonzo, J.D.
    The C-terminal end of the Trypanosoma brucei editing deaminase plays a critical role in tRNA binding (2011), RNA, 17, 1296-1306.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.33
-
 Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.33 C-terDELTA10 mutation in subunit ADAT2, inactive mutant enzyme Trypanosoma brucei
3.5.4.33 C-terDELTA5 mutation in subunit ADAT2, kcat/Km is 72% compared to the wild-type value Trypanosoma brucei
3.5.4.33 E92A mutation in subunit ADAT2, inactive mutant enzyme Trypanosoma brucei
3.5.4.33 E92A/K216A/R217A/K218A/R219A inactive mutant enzyme Trypanosoma brucei
3.5.4.33 K216A mutation in subunit ADAT2, kcat/Km is 12% compared to the wild-type value Trypanosoma brucei
3.5.4.33 K218A mutation in subunit ADAT2, kcat/Km is 24% compared to the wild-type value Trypanosoma brucei
3.5.4.33 additional information deletion of the last 10 amino acids at the C terminus of TbADAT2 abolishes tRNA binding. In addition, single alanine replacements of a string of positively charged amino acids (KRKRK) lead to binding defects that correlate with losses in enzyme activity Trypanosoma brucei
3.5.4.33 R217A mutation in subunit ADAT2, kcat/Km is 16% compared to the wild-type value Trypanosoma brucei
3.5.4.33 R219A mutation in subunit ADAT2, kcat/Km is 20% compared to the wild-type value Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.33 0.00075
-
 adenine34 in tRNAVal 27°C, pH 8.0, wild-type enzyme Trypanosoma brucei
3.5.4.33 0.00076
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme C-terDELTA5, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 0.002
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme R218A Trypanosoma brucei
3.5.4.33 0.0025
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme R217A Trypanosoma brucei
3.5.4.33 0.0028
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme R219A Trypanosoma brucei
3.5.4.33 0.003
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme K216A Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.33 Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.33 adenine34 in tRNAVal + H2O
-
 Trypanosoma brucei hypoxanthine34 in tRNAVal + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.33 27
-
 assay at Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.33 0.0015
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme R217A Trypanosoma brucei
3.5.4.33 0.0017
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme K216A Trypanosoma brucei
3.5.4.33 0.002
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme R218A Trypanosoma brucei
3.5.4.33 0.0022
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme R219A Trypanosoma brucei
3.5.4.33 0.0023
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme C-terDELTA5, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 0.0032
-
 adenine34 in tRNAVal 27°C, pH 8.0, wild-type enzyme Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.33 8
-
 assay at Trypanosoma brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.4.33 0.57
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme K216A Trypanosoma brucei
3.5.4.33 0.59
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme R217A Trypanosoma brucei
3.5.4.33 0.79
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme R219A Trypanosoma brucei
3.5.4.33 1
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme R218A Trypanosoma brucei
3.5.4.33 3.03
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme C-terDELTA5, mutation in subunit ADAT2 Trypanosoma brucei
3.5.4.33 4.27
-
 adenine34 in tRNAVal 27°C, pH 8.0, wild-type enzyme Trypanosoma brucei