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Literature summary extracted from

  • Cobzaru, C.; Ganas, P.; Mihasan, M.; Schleberger, P.; Brandsch, R.
    Homologous gene clusters of nicotine catabolism, including a new omega-amidase for alpha-ketoglutaramate, in species of three genera of Gram-positive bacteria (2011), Res. Microbiol., 162, 285-291.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.3 gene nit, the gene is encoded in the nic-gene cluster located on plasmid pAO1 and flanked by mobile elements Paenarthrobacter nicotinovorans
3.5.1.3 the gene is encoded in the nic-gene cluster on the chromosome Rhodococcus opacus
3.5.1.111 expressed in Escherichia coli Paenarthrobacter nicotinovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.111 6.3
-
2-Oxoglutaramate pH 7.1, 30 °C Paenarthrobacter nicotinovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.111 32300
-
calculated from cDNA Paenarthrobacter nicotinovorans
3.5.1.111 137000
-
gel filtration Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.3 2-oxoglutaramate + H2O Rhodococcus opacus
-
2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O Nocardioides sp.
-
2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O Paenarthrobacter nicotinovorans
-
2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O Nocardioides sp. JS614 / ATCC BAA-499
-
2-oxoglutarate + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.3 Nocardioides sp.
-
-
-
3.5.1.3 Nocardioides sp. JS614 / ATCC BAA-499
-
-
-
3.5.1.3 Paenarthrobacter nicotinovorans
-
gene nit
-
3.5.1.3 Rhodococcus opacus
-
-
-
3.5.1.111 Paenarthrobacter nicotinovorans Q93NG1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.3 2-oxoglutaramate + H2O
-
Rhodococcus opacus 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O
-
Nocardioides sp. 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O
-
Paenarthrobacter nicotinovorans 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O the conserved Cys-Glu-Lys triad is involved in catalysis Paenarthrobacter nicotinovorans 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O
-
Nocardioides sp. JS614 / ATCC BAA-499 2-oxoglutarate + NH3
-
?
3.5.1.111 2-oxoglutaramate + H2O
-
Paenarthrobacter nicotinovorans ?
-
?
3.5.1.111 L-glutamic acid + H2O poor substrate Paenarthrobacter nicotinovorans ?
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.111 homotetramer 4 * 32300, gel filtration Paenarthrobacter nicotinovorans

Synonyms

EC Number Synonyms Comment Organism
3.5.1.111 omega-amidase
-
Paenarthrobacter nicotinovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.3 30
-
assay at Rhodococcus opacus
3.5.1.3 30
-
assay at Nocardioides sp.
3.5.1.3 30
-
assay at Paenarthrobacter nicotinovorans
3.5.1.111 30
-
assay at Paenarthrobacter nicotinovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.111 194
-
2-Oxoglutaramate pH 7.1, 30 °C Paenarthrobacter nicotinovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.3 7.1
-
assay at Rhodococcus opacus
3.5.1.3 7.1
-
assay at Nocardioides sp.
3.5.1.3 7.1
-
assay at Paenarthrobacter nicotinovorans
3.5.1.111 7.1
-
assay at Paenarthrobacter nicotinovorans

General Information

EC Number General Information Comment Organism
3.5.1.3 metabolism the omega-amidase is involved in the nicotine catabolism, and encoded in organized clusters of homologous genes for nicotine catabolism. The omega-amidase, together with glutamate dehydrogenase, may form a physiologically relevant enzyme couple, leading to transformation of metabolically inert alpha-oxoglutaramate derived from trihydroxypyridine into glutamate, a central compound of nitrogen metabolism Rhodococcus opacus
3.5.1.3 metabolism the omega-amidase is involved in the nicotine catabolism, and encoded in organized clusters of homologous genes for nicotine catabolism. The omega-amidase, together with glutamate dehydrogenase, may form a physiologically relevant enzyme couple, leading to transformation of metabolically inert alpha-oxoglutaramate derived from trihydroxypyridine into glutamate, a central compound of nitrogen metabolism Nocardioides sp.
3.5.1.3 metabolism the omega-amidase is involved in the nicotine catabolism, and encoded in organized clusters of homologous genes for nicotine catabolism. The omega-amidase, together with glutamate dehydrogenase, may form a physiologically relevant enzyme couple, leading to transformation of metabolically inert alpha-oxoglutaramate derived from trihydroxypyridine into glutamate, a central compound of nitrogen metabolism Paenarthrobacter nicotinovorans
3.5.1.3 physiological function the omega-amidase, together with glutamate dehydrogenase, may form a physiologically relevant enzyme couple, leading to transformation of metabolically inert alpha-oxoglutaramate derived from trihydroxypyridine into glutamate, a central compound of nitrogen metabolism Rhodococcus opacus
3.5.1.3 physiological function the omega-amidase, together with glutamate dehydrogenase, may form a physiologically relevant enzyme couple, leading to transformation of metabolically inert alpha-oxoglutaramate derived from trihydroxypyridine into glutamate, a central compound of nitrogen metabolism Nocardioides sp.
3.5.1.3 physiological function the omega-amidase, together with glutamate dehydrogenase, may form a physiologically relevant enzyme couple, leading to transformation of metabolically inert alpha-oxoglutaramate derived from trihydroxypyridine into glutamate, a central compound of nitrogen metabolism Paenarthrobacter nicotinovorans