EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.3 | gene nit, the gene is encoded in the nic-gene cluster located on plasmid pAO1 and flanked by mobile elements | Paenarthrobacter nicotinovorans |
3.5.1.3 | the gene is encoded in the nic-gene cluster on the chromosome | Rhodococcus opacus |
3.5.1.111 | expressed in Escherichia coli | Paenarthrobacter nicotinovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.111 | 6.3 | - |
2-Oxoglutaramate | pH 7.1, 30 °C | Paenarthrobacter nicotinovorans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.111 | 32300 | - |
calculated from cDNA | Paenarthrobacter nicotinovorans |
3.5.1.111 | 137000 | - |
gel filtration | Paenarthrobacter nicotinovorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.3 | 2-oxoglutaramate + H2O | Rhodococcus opacus | - |
2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | Nocardioides sp. | - |
2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | Paenarthrobacter nicotinovorans | - |
2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | Nocardioides sp. JS614 / ATCC BAA-499 | - |
2-oxoglutarate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.3 | Nocardioides sp. | - |
- |
- |
3.5.1.3 | Nocardioides sp. JS614 / ATCC BAA-499 | - |
- |
- |
3.5.1.3 | Paenarthrobacter nicotinovorans | - |
gene nit | - |
3.5.1.3 | Rhodococcus opacus | - |
- |
- |
3.5.1.111 | Paenarthrobacter nicotinovorans | Q93NG1 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.3 | 2-oxoglutaramate + H2O | - |
Rhodococcus opacus | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | - |
Nocardioides sp. | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | - |
Paenarthrobacter nicotinovorans | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | the conserved Cys-Glu-Lys triad is involved in catalysis | Paenarthrobacter nicotinovorans | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | - |
Nocardioides sp. JS614 / ATCC BAA-499 | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.111 | 2-oxoglutaramate + H2O | - |
Paenarthrobacter nicotinovorans | ? | - |
? | |
3.5.1.111 | L-glutamic acid + H2O | poor substrate | Paenarthrobacter nicotinovorans | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.111 | homotetramer | 4 * 32300, gel filtration | Paenarthrobacter nicotinovorans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.111 | omega-amidase | - |
Paenarthrobacter nicotinovorans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.3 | 30 | - |
assay at | Rhodococcus opacus |
3.5.1.3 | 30 | - |
assay at | Nocardioides sp. |
3.5.1.3 | 30 | - |
assay at | Paenarthrobacter nicotinovorans |
3.5.1.111 | 30 | - |
assay at | Paenarthrobacter nicotinovorans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.111 | 194 | - |
2-Oxoglutaramate | pH 7.1, 30 °C | Paenarthrobacter nicotinovorans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.3 | 7.1 | - |
assay at | Rhodococcus opacus |
3.5.1.3 | 7.1 | - |
assay at | Nocardioides sp. |
3.5.1.3 | 7.1 | - |
assay at | Paenarthrobacter nicotinovorans |
3.5.1.111 | 7.1 | - |
assay at | Paenarthrobacter nicotinovorans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.1.3 | metabolism | the omega-amidase is involved in the nicotine catabolism, and encoded in organized clusters of homologous genes for nicotine catabolism. The omega-amidase, together with glutamate dehydrogenase, may form a physiologically relevant enzyme couple, leading to transformation of metabolically inert alpha-oxoglutaramate derived from trihydroxypyridine into glutamate, a central compound of nitrogen metabolism | Rhodococcus opacus |
3.5.1.3 | metabolism | the omega-amidase is involved in the nicotine catabolism, and encoded in organized clusters of homologous genes for nicotine catabolism. The omega-amidase, together with glutamate dehydrogenase, may form a physiologically relevant enzyme couple, leading to transformation of metabolically inert alpha-oxoglutaramate derived from trihydroxypyridine into glutamate, a central compound of nitrogen metabolism | Nocardioides sp. |
3.5.1.3 | metabolism | the omega-amidase is involved in the nicotine catabolism, and encoded in organized clusters of homologous genes for nicotine catabolism. The omega-amidase, together with glutamate dehydrogenase, may form a physiologically relevant enzyme couple, leading to transformation of metabolically inert alpha-oxoglutaramate derived from trihydroxypyridine into glutamate, a central compound of nitrogen metabolism | Paenarthrobacter nicotinovorans |
3.5.1.3 | physiological function | the omega-amidase, together with glutamate dehydrogenase, may form a physiologically relevant enzyme couple, leading to transformation of metabolically inert alpha-oxoglutaramate derived from trihydroxypyridine into glutamate, a central compound of nitrogen metabolism | Rhodococcus opacus |
3.5.1.3 | physiological function | the omega-amidase, together with glutamate dehydrogenase, may form a physiologically relevant enzyme couple, leading to transformation of metabolically inert alpha-oxoglutaramate derived from trihydroxypyridine into glutamate, a central compound of nitrogen metabolism | Nocardioides sp. |
3.5.1.3 | physiological function | the omega-amidase, together with glutamate dehydrogenase, may form a physiologically relevant enzyme couple, leading to transformation of metabolically inert alpha-oxoglutaramate derived from trihydroxypyridine into glutamate, a central compound of nitrogen metabolism | Paenarthrobacter nicotinovorans |