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Literature summary extracted from

  • Mathews, I.; Schwarzenbacher, R.; McMullan, D.; Abdubek, P.; Ambing, E.; Axelrod, H.; Biorac, T.; Canaves, J.; Chiu, H.; Deacon, A.; DiDonato, M.; Elsliger, M.; Godzik, A.; Grittini, C.; Grzechnik, S.; Hale, J.; Hampton, E.; Han, G.; Haugen, J.; Hornsby,
    Crystal structure of S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA) from Thermotoga maritima at 2.0 A resolution reveals a new fold (2005), Proteins, 59, 869-874.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.99.17 expressed in Escherichia coli strain DL41 Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.99.17 nanodroplet vapor diffusion method, using 12% (w/v) polyethylene glycol 4000, 0.1 M citric acid at pH 5.0 Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.99.17 38529
-
x * 38529, calculated from amino acid sequence Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.17 Thermotoga maritima Q9WZ44
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.99.17 nickel-resin column chromatography, RESOURCE Q column chromatography, and Superdex 200 gel filtration Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.17 S-adenosyl-L-methionine + 7-aminomethyl-7-deazaguanine34 in tRNA the enzyme catalyzes the formation of the 2,3-epoxy-4,5-dihydroxycyclopentane ring of the queuosine precursor epoxyqueuosine Thermotoga maritima L-methionine + adenine + epoxyqueuosine34 in tRNA
-
?

Subunits

EC Number Subunits Comment Organism
2.4.99.17 ? x * 38529, calculated from amino acid sequence Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.4.99.17 QueA
-
Thermotoga maritima
2.4.99.17 queuosine biosynthesis protein
-
Thermotoga maritima
2.4.99.17 S-adenosylmethionine:tRNA ribosyltransferase-isomerase
-
Thermotoga maritima
2.4.99.17 TM0574
-
Thermotoga maritima

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.99.17 Thermotoga maritima calculated from amino acid sequence
-
8.6