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Literature summary extracted from

  • Gehret, J.J.; Gu, L.; Geders, T.W.; Brown, W.C.; Gerwick, L.; Gerwick, W.H.; Sherman, D.H.; Smith, J.L.
    Structure and activity of DmmA, a marine haloalkane dehalogenase (2012), Protein Sci., 21, 239-248.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.5 gene dmmA from cosmid pLM17, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutants in Escherichia coli strain BL21(DE3) uncultured marine bacterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.8.1.5 purified recombinant selenomethionyl and bative DmmAshort and native DmmAlong form, vapor diffusion, for the short form: mixing of equal volumes of protein solution containing 6 mg/mL DmmAshort in 20 mM Tris, pH 7.9, 500 mM NaCl, 10% glycerol, with well solution containing 2.2 M sodium malonate, pH 7.0, 5% glycerol, 20°C, 3-4 days, for the long form: mixing of equal volumes of protein solution containing 14 mg/mL DmmAlong in 20 mM Tris, pH 7.9, 500 mM NaCl, 10% glycerol, with well solution containing 0.6 M lithium sulfate, 27.5% PEG3350, and 0.1 M BisTris, pH 5.5, cryoprotection by 25% PEG400, X-ray diffraction structure determination and analysis at 2.2 A and 2.9 A resolution, respectively uncultured marine bacterium

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.5 additional information generation of a truncated enzyme form lacking the N-terminus, DmmAshort, from full-length wild-type enzyme, DmmAlong uncultured marine bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.5 additional information
-
 additional information steady-state kinetics, overview uncultured marine bacterium
3.8.1.5 0.24
-
 1,3-dibromopropane pH and temperature not specified in the publication uncultured marine bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.5 1,3-dibromopropane + H2O uncultured marine bacterium
-
 3-bromopropanol + bromide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 no activity in Moorea producta
-
 formerly Lyngbya majuscula
-
3.8.1.5 uncultured marine bacterium
-
 gene dmmA, formerly curN, from cosmid pLM17
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.5 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration uncultured marine bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 1,3-dibromopropane + H2O
-
 uncultured marine bacterium 3-bromopropanol + bromide
-
 ?
3.8.1.5 1,3-dibromopropane + H2O preferred substrate uncultured marine bacterium 3-bromopropanol + bromide
-
 ?
3.8.1.5 1,6-dibromohexane + H2O low activity uncultured marine bacterium 6-bromohexanol + bromide
-
 ?
3.8.1.5 1,6-dichlorohexane + H2O low activity uncultured marine bacterium 6-chlorohexanol + chloride
-
 ?
3.8.1.5 1,6-diiodohexane + H2O low activity uncultured marine bacterium 6-iodohexane + iodide
-
 ?
3.8.1.5 bromocyclohexane + H2O
-
 uncultured marine bacterium cyclohexanol + bromide
-
 ?
3.8.1.5 additional information substrate specificity, overview uncultured marine bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.5 monomer or dimer dynamic equilibrium between monomeric and dimeric forms uncultured marine bacterium
3.8.1.5 More DmmA adopts the alpha/beta hydrolase fold, consisting of core and lid sub-domains with the active site at the top of the core, which is covered by the lid, quaternary structure comparisons, overview uncultured marine bacterium

Synonyms

EC Number Synonyms Comment Organism
3.8.1.5 DmmA formerly CurN uncultured marine bacterium
3.8.1.5 haloalkane dehalogenase
-
 uncultured marine bacterium
3.8.1.5 HLD
-
 uncultured marine bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8.1.5 2.4
-
 1,3-dibromopropane pH and temperature not specified in the publication uncultured marine bacterium

General Information

EC Number General Information Comment Organism
3.8.1.5 evolution DmmA belongs to the haloalkane dehalogenase family, subfamily-II, structure comparisons, overview uncultured marine bacterium
3.8.1.5 additional information the active site is formed by a pentad consisting of halide-stabilizing Asn78 and Trp145, nucleophile Asp144, base His315, and acid Glu168. DmmA possesses a larg active site binding pocket allowing to accept a range of linear and cyclic substrates including include chlorinated, brominated, and iodinated alkanes of varying length, structure, overview uncultured marine bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.8.1.5 10
-
 1,3-dibromopropane pH and temperature not specified in the publication uncultured marine bacterium