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Literature summary extracted from

  • Okai, M.; Ohtsuka, J.; Asano, A.; Guo, L.; Miyakawa, T.; Miyazono, K.; Nakamura, A.; Okada, A.; Zheng, H.; Kimura, K.; Nagata, K.; Tanokura, M.
    High pressure refolding, purification, and crystallization of flavin reductase from Sulfolobus tokodaii strain 7 (2012), Protein Expr. Purif., 84, 214-218.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.36 expressed in Escherichia coli Rosetta(DE3) cells Sulfurisphaera tokodaii
1.5.1.41 expressed of N-terminally His-tagged HpaCSt protein in Escherichia coli Sulfurisphaera tokodaii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.36 complexed with FMN, FAD or riboflavin, sitting drop vapor diffusion method, using 1.6 M ammonium sulfate and 4-5% (v/v) isopropanol Sulfurisphaera tokodaii
1.5.1.41 crystals of the refolded enzyme are obtained by adding FMN, FAD, or riboflavin to the protein solution and without the addition of flavin compound Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.36 Sulfurisphaera tokodaii
-
-
-
1.5.1.36 Sulfurisphaera tokodaii 7
-
-
-
1.5.1.41 Sulfurisphaera tokodaii
-
-
-
1.5.1.41 Sulfurisphaera tokodaii 7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.36 Ni-affinity chromatography and heat treatment Sulfurisphaera tokodaii
1.5.1.41
-
 Sulfurisphaera tokodaii

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.5.1.36
-
 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.36 FMN + NADH
-
 Sulfurisphaera tokodaii FMNH2 + NAD+
-
 ?
1.5.1.36 FMN + NADH
-
 Sulfurisphaera tokodaii 7 FMNH2 + NAD+
-
 ?
1.5.1.41 FMN + NADH + H+
-
 Sulfurisphaera tokodaii FMNH2 + NAD+
-
 ?
1.5.1.41 FMN + NADH + H+
-
 Sulfurisphaera tokodaii 7 FMNH2 + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.36 HpaC
-
 Sulfurisphaera tokodaii
1.5.1.41 flavin reductase HpaCSt
-
 Sulfurisphaera tokodaii

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.36 NADH
-
 Sulfurisphaera tokodaii
1.5.1.36 NADPH
-
 Sulfurisphaera tokodaii
1.5.1.41 FMN the purified refolded enzyme does not contain FMN, unlike the same enzyme expressed as a soluble protein. After the addition of FMN to the protein solution, the refolded enzyme shows a higher activity than the enzyme expressed as the soluble protein Sulfurisphaera tokodaii