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Literature summary extracted from

  • Pokrovskaya, M.V.; Aleksandrova, S.S.; Pokrovsky, V.S.; Omeljanjuk, N.M.; Borisova, A.A.; Anisimova, N.Y.; Sokolov, N.N.
    Cloning, expression and characterization of the recombinant Yersinia pseudotuberculosis L-asparaginase (2012), Protein Expr. Purif., 82, 150-154.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 gene ansB, expression in Escherichia coli strain BL21 (DE3) Yersinia pseudotuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.017
-
 L-asparagine pH 8.0, 37°C Yersinia pseudotuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 KCl the catalytic activity of YpA does not vary significantly as a function of ionic strength at 100-3000 mM KCl Yersinia pseudotuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Yersinia pseudotuberculosis
-
 L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O Yersinia pseudotuberculosis IP32953
-
 L-aspartate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Yersinia pseudotuberculosis Q66CJ2 gene ansB
-
3.5.1.1 Yersinia pseudotuberculosis IP32953 Q66CJ2 gene ansB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 recombinant YpA from Escherichia coli strain BL21 (DE3) by two different steps of anion exchange chromatography Yersinia pseudotuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 62.7
-
 pH 8.0, 37°C, purified enzyme Yersinia pseudotuberculosis

Storage Stability

EC Number Storage Stability Organism
3.5.1.1 -70°C to -20°C, purified enzyme, pH 5.0-9.0, several weeks, no loss of activity Yersinia pseudotuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Yersinia pseudotuberculosis L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Yersinia pseudotuberculosis IP32953 L-aspartate + NH3
-
 ?
3.5.1.1 L-glutamine + H2O YpA L-glutaminase activity is relatively low and more than 15 times less than specific activity towards L-Asn Yersinia pseudotuberculosis L-glutamate + NH3
-
 ?
3.5.1.1 L-glutamine + H2O YpA L-glutaminase activity is relatively low and more than 15 times less than specific activity towards L-Asn Yersinia pseudotuberculosis IP32953 L-glutamate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 L-asparaginase
-
 Yersinia pseudotuberculosis
3.5.1.1 Ypa
-
 Yersinia pseudotuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 60
-
-
 Yersinia pseudotuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 57 73 over 80% of maximal activity within this range Yersinia pseudotuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 60
-
 purified enzyme, pH 5.0-9.0, 15% activity remaining after 3 min, 6% after 5 min Yersinia pseudotuberculosis
3.5.1.1 80
-
 purified enzyme, pH 5.0-9.0, below 1% activity remaining after 10 min Yersinia pseudotuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 0.22
-
 L-asparagine pH 8.0, 37°C Yersinia pseudotuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 8
-
-
 Yersinia pseudotuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 6 9 over 80% of maximal activity within this range Yersinia pseudotuberculosis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.1 Yersinia pseudotuberculosis isoelectric focusing
-
 5.4

General Information

EC Number General Information Comment Organism
3.5.1.1 physiological function antiproliferative effect of YpA in vitro and in vivo, overview Yersinia pseudotuberculosis