EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.258 | expressed in Escherichia coli Rosetta (DE3) pLysS cells | Clostridioides difficile |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.258 | 0.058 | - |
hydroxycobalamin | recombinant enzyme, in 0.1 M MES, at pH 5.1 and 25°C | Clostridioides difficile |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.258 | 30000 | - |
2 * 30000, SDS-PAGE | Clostridioides difficile |
2.1.1.258 | 31000 | - |
2 * 31000, calculated from amino acid sequence | Clostridioides difficile |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.258 | Clostridioides difficile | - |
- |
- |
2.1.1.258 | Clostridioides difficile 630 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.258 | amylose resin column chromatography, Ni-NTA column chromatography, Sephadex-G25 gel filtration, and Superdex 75 gel filtration | Clostridioides difficile |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.258 | hydroxycobalamin + 5-methyltetrahydrofolate | - |
Clostridioides difficile | methylcobalamin + tetrahydrofolate | - |
? | |
2.1.1.258 | hydroxycobalamin + 5-methyltetrahydrofolate | - |
Clostridioides difficile 630 | methylcobalamin + tetrahydrofolate | - |
? | |
2.1.1.258 | [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate | - |
Clostridioides difficile | [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate | - |
? | |
2.1.1.258 | [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate | - |
Clostridioides difficile 630 | [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.258 | homodimer | 2 * 30000, SDS-PAGE | Clostridioides difficile |
2.1.1.258 | homodimer | 2 * 31000, calculated from amino acid sequence | Clostridioides difficile |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.258 | methyltetrahydrofolate- and corrinoid-dependent methyltransferase | - |
Clostridioides difficile |
2.1.1.258 | MeTr | - |
Clostridioides difficile |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.258 | 16 | - |
the recombinant enzyme is not stable at 16°C or higher temperature | Clostridioides difficile |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.258 | 77.6 | - |
hydroxycobalamin | recombinant enzyme, in 0.1 M MES, at pH 5.1 and 25°C | Clostridioides difficile |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.258 | 1330 | - |
hydroxycobalamin | recombinant enzyme, in 0.1 M MES, at pH 5.1 and 25°C | Clostridioides difficile |