EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.48 | expression of isoform a in Escherichia coli | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.48 | in complex with acetyl coenzyme A and histone H4 peptide, i.e. residues 1-20, to 1.9 A resolution. The cofactor and the side chain of lysine 12 of histone H4 peptide are placed in the canyon between the central and C-terminal domains. Histone H4 peptide adopts a well-defined conformation and establishes an extensive set of interactions with the enzyme including invariant residues Glu64 and Trp199, which together govern substrate-binding specificity. There is a cumulative effect of the active-site residues Glu187, Glu276, and Asp277 on deprotonation of the epsilon-amino group of reactive Lys12 for direct attack of the acetyl group of the cofactor | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.48 | D277N | strong decrease in catalytic efficiency | Homo sapiens |
2.3.1.48 | D62A | 4fold increase in Km value | Homo sapiens |
2.3.1.48 | E187Q | strong decrease in catalytic efficiency | Homo sapiens |
2.3.1.48 | E276Q | strong decrease in catalytic efficiency | Homo sapiens |
2.3.1.48 | E64A | 6fold increase in Km value | Homo sapiens |
2.3.1.48 | W199A | 3fold increase in Km value | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.48 | 0.0067 | - |
acetyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 0.0208 | - |
histone H4 peptide | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 0.0595 | - |
histone H4 peptide | mutant W199A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 0.086 | - |
histone H4 peptide | mutant D62A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 0.099 | - |
histone H4 peptide | mutant D277N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 0.1 | - |
histone H4 peptide | mutant E276Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 0.1267 | - |
histone H4 peptide | mutant E64A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 0.197 | - |
histone H4 peptide | mutant E187Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.48 | Homo sapiens | O14929 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.48 | acetyl-CoA + histone H4 peptide | residues 1-20 of histone H4 | Homo sapiens | CoA + acetylpeptide of histone H4 | - |
? | |
2.3.1.48 | additional information | Glu187 and Glu276 could act as the general catalytic base and together with Asp277 have a cumulative effect on deprotonation of the ?-amino group of substrate Lys12 of the histone H4 peptide, which could be mediated by water molecules | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.48 | Hat1 | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.48 | 0.15 | - |
histone H4 peptide | mutant E276Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 0.28 | - |
histone H4 peptide | mutant E187Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 0.41 | - |
histone H4 peptide | mutant W199A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 0.5 | - |
histone H4 peptide | mutant E64A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 0.53 | - |
histone H4 peptide | mutant D277N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 1.99 | - |
histone H4 peptide | mutant D62A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 4.14 | - |
acetyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 4.28 | - |
histone H4 peptide | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.48 | 1.41 | - |
histone H4 peptide | mutant E187Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 1.5 | - |
histone H4 peptide | mutant E276Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 3.95 | - |
histone H4 peptide | mutant E64A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 4.88 | - |
histone H4 peptide | mutant W199A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 5.36 | - |
histone H4 peptide | mutant D277N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 23.9 | - |
histone H4 peptide | mutant D62A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 205 | - |
histone H4 peptide | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
2.3.1.48 | 619 | - |
acetyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |