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Literature summary extracted from

  • Boerud, B.; Viburiene, R.; Hartley, M.D.; Paulsen, B.S.; Egge-Jacobsen, W.; Imperiali, B.; Koomey, M.
    Genetic and molecular analyses reveal an evolutionary trajectory for glycan synthesis in a bacterial protein glycosylation system (2011), Proc. Natl. Acad. Sci. USA, 108, 9643-9648.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.292 H371R site-directed mutagenesis, strain FA 1090, the mutation is sufficient to restore PglH activity in the wild-type FA1090 strain Neisseria gonorrhoeae
2.4.1.292 additional information replacement of the strain N400 locus carrying the deleted form of ORFs 2 and 3 with those derived from the Neisseria gonorrhoeae strain FA1090 and from diverse neisserial strains into Neisseria gonorrhoeae strain N400 in which the pgl gene function and associated glycan structures is defined, mutant glycosylation patterns, overview. The presence of a stereotypic, conserved deletion mutation is inactivating pglH in strains of Neisseria gonorrhoeae, Neisseria meningitidis, and related commensals Neisseria gonorrhoeae
2.4.1.292 additional information replacement of the strain N400 locus carrying the deleted form of ORFs 2 and 3 with those derived from the Neisseria gonorrhoeae strain FA1090. The presence of a stereotypic, conserved deletion mutation is inactivating pglH in strains of Neisseria gonorrhoeae, Neisseria meningitidis, and related commensals Neisseria meningitidis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.292 3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol Neisseria gonorrhoeae
-
 3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
-
 ?
2.4.1.292 3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol Neisseria meningitidis
-
 3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
-
 ?
2.4.1.292 additional information Neisseria meningitidis PglH (from ORF3) acts as a glucosyltransferase, which uses both undecaprenyl-diphospho-linked Bac2,4diNAc and glyceramido-acetamido trideoxyhexose, GATDH, glycosyltransferase specificity of PglH, overview. Recombinantly expressed PglH from strain Z2491 specifically transfers UDP-Glc to undecaprenyldiphospho-Bac2,4diNAc. PglH shows no transferase activity in the presence of UDP-Gal, UDP-GlcNAc, UDPGalNAc, and GDP-Man ?
-
 ?
2.4.1.292 additional information Neisseria gonorrhoeae PglH (from ORF3) acts as a glucosyltransferase, which uses both UndPP-linked diNAcBac and glyceramido-acetamido trideoxyhexose, GATDH ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.292 Neisseria gonorrhoeae
-
 strains FA1090 and N400, gene pglH
-
2.4.1.292 Neisseria meningitidis
-
 strains FAM18 and Z2491, gene pglH
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.292 3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
-
 Neisseria gonorrhoeae 3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
-
 ?
2.4.1.292 3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
-
 Neisseria meningitidis 3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol
-
 ?
2.4.1.292 additional information PglH (from ORF3) acts as a glucosyltransferase, which uses both undecaprenyl-diphospho-linked Bac2,4diNAc and glyceramido-acetamido trideoxyhexose, GATDH, glycosyltransferase specificity of PglH, overview. Recombinantly expressed PglH from strain Z2491 specifically transfers UDP-Glc to undecaprenyldiphospho-Bac2,4diNAc. PglH shows no transferase activity in the presence of UDP-Gal, UDP-GlcNAc, UDPGalNAc, and GDP-Man Neisseria meningitidis ?
-
 ?
2.4.1.292 additional information PglH (from ORF3) acts as a glucosyltransferase, which uses both UndPP-linked diNAcBac and glyceramido-acetamido trideoxyhexose, GATDH Neisseria gonorrhoeae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.292 PglH
-
 Neisseria gonorrhoeae
2.4.1.292 PglH
-
 Neisseria meningitidis

General Information

EC Number General Information Comment Organism
2.4.1.292 malfunction polymorphisms in PglH are associated with diminished glycosyltransferase activity Neisseria meningitidis
2.4.1.292 additional information Pgl genotypes and phenotypes of strains, overview Neisseria meningitidis
2.4.1.292 additional information Pgl genotypes and phenotypes of strains, overview. Epistatic interactions involving pglH at the intragenic and intergenic levels Neisseria gonorrhoeae
2.4.1.292 physiological function metabolic conflict related to competition for a shared substrate between the opposing glycosyltransferases PglA and PglH Neisseria meningitidis
2.4.1.292 physiological function metabolic conflict related to competition for a shared substrate between the opposing glycosyltransferases PglA and PglH. Protein-linked PglH-derived disaccharides are immunogenic and antigenic, both diNAcBac- and GATDH-based PglH-derived glycans act as glycosyl donors in general, broad-spectrum protein glycosylation Neisseria gonorrhoeae