Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Goulas, T.; Arolas, J.L.; Gomis-Rueth, F.X.
    Structure, function and latency regulation of a bacterial enterotoxin potentially derived from a mammalian adamalysin/ADAM xenolog (2011), Proc. Natl. Acad. Sci. USA, 108, 1856-1861.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.74 expressed in Escherichia coli Origami-2 (DE3) cells Bacteroides fragilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.74 sitting drop vapor diffusion method, using 100 mM sodium citrate dihydrate, 20% (w/v) PEG 3000, pH 5.5 Bacteroides fragilis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.74 E349A residual activity against fibrinogen and fluorophore-labeled casein Bacteroides fragilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.74 CT1746
-
 Bacteroides fragilis
3.4.24.74 additional information activity is completely abolished by standard zinc-chelating agents Bacteroides fragilis
3.4.24.74 Zn2+ activity is significantly impaired by excess zinc Bacteroides fragilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.74 Zn2+ dependent on Bacteroides fragilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.74 21000
-
 x * 21000, SDS-PAGE Bacteroides fragilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.74 E-cadherin + H2O Bacteroides fragilis in vivo substrate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.74 Bacteroides fragilis O86049
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.74 nickel-affinity column chromatography Bacteroides fragilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.74 Abz-Asp-Tyr-Val-Ala-Ser-Glu-Lys(Dnp) + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 Abz-Gly-Ile-Val-Arg-Ala-Lys(Dnp) + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 Abz-Lys-Asp-Glu-Ser-Tyr-Arg-Lys(Dnp) + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 Abz-Thr-Val-Leu-Glu-Arg-Ser-Lys(Dnp) + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 Abz-Tyr-Gly-Lys-Arg-Val-Phe-Lys(Dnp) + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 Abz-Val-Lys-Phe-Tyr-Asp-Ile-Lys(Dnp) + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 actin + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 azocasein + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 azocollagen + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 BODIPY FL-casein + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 casein + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 E-cadherin + H2O in vivo substrate Bacteroides fragilis ?
-
 ?
3.4.24.74 Fibrinogen + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 Fibronectin + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 + H2O
-
 Bacteroides fragilis ?
-
 ?
3.4.24.74 additional information isozyme fargilysin-3 does not cleave azoalbumin Bacteroides fragilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.74 ? x * 21000, SDS-PAGE Bacteroides fragilis

Synonyms

EC Number Synonyms Comment Organism
3.4.24.74 Bacteroides fragilis toxin
-
 Bacteroides fragilis
3.4.24.74 BFT-3 isozyme Bacteroides fragilis
3.4.24.74 fragilysin-3 isozyme Bacteroides fragilis