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Literature summary extracted from

  • Shida, T.; Cueva, J.G.; Xu, Z.; Goodman, M.B.; Nachury, M.V.
    The major alpha-tubulin K40 acetyltransferase alphaTAT1 promotes rapid ciliogenesis and efficient mechanosensation (2010), Proc. Natl. Acad. Sci. USA, 107, 21517-21522.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.108 D157N inactive mutant Caenorhabditis elegans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.108 microtubule acetylation of alpha-tubulin K40 by alphaTAT1 inside the microtubule lumen Caenorhabditis elegans 5874
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.108 acetyl-CoA + [alpha-tubulin]-L-lysine Caenorhabditis elegans K40 of alpha-tubulin is the sole site of acetylation by alphaTAT1, alphaTAT1 specifically acetylates K40 of alpha-tubulin and prefers microtubules over free tubulin, overview CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.108 Caenorhabditis elegans
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2.3.1.108 no activity in Potorous tridactylis
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.108 additional information robust alpha-tubulin K40 acetylation in the touch receptor neurons Caenorhabditis elegans
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.108 acetyl-CoA + [alpha-tubulin]-L-lysine K40 of alpha-tubulin is the sole site of acetylation by alphaTAT1, alphaTAT1 specifically acetylates K40 of alpha-tubulin and prefers microtubules over free tubulin, overview Caenorhabditis elegans CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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 ?
2.3.1.108 acetyl-CoA + [alpha-tubulin]-L-lysine alphaTAT1 acetylates tubulin through its GNAT domain in vitro. K40 of alpha-tubulin is the sole site of acetylation by alphaTAT1. alphaTAT1 displays a greater catalytic efficiency for taxol-stabilized microtubules than for free tubulin Caenorhabditis elegans CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.108 alpha-tubulin K40 acetyltransferase
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 Caenorhabditis elegans
2.3.1.108 alphaTAT1
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 Caenorhabditis elegans
2.3.1.108 alphaTAT2
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 Caenorhabditis elegans
2.3.1.108 Mec-17
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 Caenorhabditis elegans

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.108 acetyl-CoA
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 Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
2.3.1.108 evolution broad phylogenetic distribution of alphaTAT1 Caenorhabditis elegans
2.3.1.108 malfunction in animals lacking MEC-17, alphaTAT-2, and the sole Caenorhabditis elegans K40 alpha-tubulin MEC-12, touch sensation can be restored by expression of an acetyl-mimic MEC-12 K40Q. Transient overexpression of alphaTAT1 in PtK2 cells is sufficient to acetylate nearly all microtubules, whereas catalytically inactive alphaTAT1 D157N or the HAT Elp3 fail to detectably elevate alpha-tubulin K40 acetylation Caenorhabditis elegans
2.3.1.108 physiological function alphaTAT1 is the major and possibly the sole alpha-tubulin K40 acetyltransferase in mammals and nematodes, and tubulin acetylation plays a conserved role in several microtubule-based processes. The worm incorporates acetylated alpha-tubulin into their microtubules. alphaTAT1 is required for the acetylation of axonemal microtubules. In Caenorhabditis elegans, microtubule acetylation is most prominent in touch receptor neurons and MEC-17, a homologue of alphaTAT1, and its paralogue alphaTAT-2 are required for alpha-tubulin acetylation and for two distinct types of touch sensation. Worm mechanosensation requires K40 alpha-tubulin acetylation Caenorhabditis elegans