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Literature summary extracted from

  • Song, E.; Rodgers, D.; Hersh, L.
    A monomeric variant of insulin degrading enzyme (IDE) loses its regulatory properties (2010), PLoS ONE, 5, e9719.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.56 ATP
-
 Rattus norvegicus
3.4.24.56 bradykinin
-
 Rattus norvegicus
3.4.24.56 dynorphin B-9
-
 Rattus norvegicus
3.4.24.56 Triphosphate
-
 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.56 additional information mutant with a deleted putative dimer interface in the C-terminal region is created, which results in a monomeric variant. Monomeric IDE retains enzymatic activity. Monomeric IDE retains, 25% of the wild type activity substrate (Abz-GGFLRKHGQ-EDDnp). With the peptide substrates beta-endorphin and amyloid beta peptide 1-40, monomeric IDE retains 1 to 0.25% of wild type activity. No activation through bradykinin or dynorphin B-9. Monomeric IDE is not activated by polyphosphates Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.56 0.0066
-
 Abz-GGFLRKHGQ-EDDnp pH 7.4, temperature not specified in the publication, mutant DELTAC lacking the C-terminal region Rattus norvegicus
3.4.24.56 0.0215
-
 Abz-GGFLRKHGQ-EDDnp pH 7.4, temperature not specified in the publication, wild-type Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.56 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.56 Abz-GGFLRKHGQ-EDDnp + H2O
-
 Rattus norvegicus Abz-GGFLR + KHGQ-EDDnp
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.56 dimer
-
 Rattus norvegicus
3.4.24.56 monomer mutant with a deleted putative dimer interface in the C-terminal region is created, which results in a monomeric variant. Monomeric IDE retains 25% of the wild type activity substrate (Abz-GGFLRKHGQ-EDDnp). With the peptide substrates beta-endorphin and amyloid beta peptide, monomeric IDE retains 1 to 0.25% of wild type activity. No activation through bradykinin or dynorphin B-9. Monomeric IDE is not activated by polyphosphates Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.24.56 IDE
-
 Rattus norvegicus
3.4.24.56 Insulysin
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.56 0.01
-
 Abz-GGFLRKHGQ-EDDnp pH 7.4, temperature not specified in the publication, mutant DELTAC lacking the C-terminal region Rattus norvegicus
3.4.24.56 0.043
-
 Abz-GGFLRKHGQ-EDDnp pH 7.4, temperature not specified in the publication, wild-type Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.56 7.4
-
 assay at Rattus norvegicus