EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.76 | expressed in Escherichia coli | Solanum habrochaites |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.76 | 3'-O-methylmyricetin | i.e. laricitrin, inhibitory at substrate concentrations above 0.025 mM | Solanum habrochaites |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.76 | 0.00055 | - |
myricetin | in 50 mM Tris-HCl (pH 7.5), at 22°C | Solanum habrochaites | |
2.1.1.76 | 0.00207 | - |
3'-O-methylmyricetin | in 50 mM Tris-HCl (pH 7.5), at 22°C | Solanum habrochaites | |
2.1.1.76 | 0.00998 | - |
7-O-methylmyricetin | in 50 mM Tris-HCl (pH 7.5), at 22°C | Solanum habrochaites | |
2.1.1.76 | 0.01086 | - |
S-adenosyl-L-methionine | in 50 mM Tris-HCl (pH 7.5), at 22°C | Solanum habrochaites |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.76 | 40000 | - |
x * 40000, SDS-PAGE | Solanum habrochaites |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.76 | additional information | Solanum habrochaites | the enzyme does not methylate 3',4',5'-O-trimethylmyricetin, 3,7,3',4'-O-methylquercetin, 3-methylquercetin, and 3,7,4'-O-trimethylkaempferol. The enzyme transfers a methyl group only to the 3-position hydroxyl | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.76 | Solanum habrochaites | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.76 | MonoQ column chromatography | Solanum habrochaites |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.76 | trichome | glandular trichome | Solanum habrochaites | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.76 | additional information | the enzyme does not methylate 3',4',5'-O-trimethylmyricetin, 3,7,3',4'-O-methylquercetin, 3-methylquercetin, and 3,7,4'-O-trimethylkaempferol. The enzyme transfers a methyl group only to the 3-position hydroxyl | Solanum habrochaites | ? | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + 3',5'-O-dimethylmyricetin | 18% activity compared to myricetin | Solanum habrochaites | S-adenosyl-L-homocysteine + 3,3',5'-O-trimethylmyricetin | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + 3'-O-methylmyricetin | 64% activity compared to myricetin | Solanum habrochaites | S-adenosyl-L-homocysteine + 3,3'-O-dimethylmyricetin | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + 3'-O-methylmyricetin | i.e. laricitrin, 43% activity compared to myricetin | Solanum habrochaites | S-adenosyl-L-homocysteine + 3,3'-O-dimethylmyricetin | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + 3-O-methylquercetin | 3% activity compared to myricetin | Solanum habrochaites | S-adenosyl-L-homocysteine + ? | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + 4'-O-methylkaempferol | 11% activity compared to myricetin | Solanum habrochaites | S-adenosyl-L-homocysteine + 3,4'-O-dimethylkaempferol | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + 7-O-methylmyricetin | 104% activity compared to myricetin | Solanum habrochaites | S-adenosyl-L-homocysteine + 3,7-O-dimethylquercetin | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + kaempferol | 53% activity compared to myricetin | Solanum habrochaites | S-adenosyl-L-homocysteine + 3-O-methylkaempferol | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + myricetin | 100% activity | Solanum habrochaites | S-adenosyl-L-homocysteine + 3-O-methylmyricetin | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + quercetin | 121% activity compared to myricetin | Solanum habrochaites | S-adenosyl-L-homocysteine + 3-O-methylquercetin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.76 | ? | x * 40000, SDS-PAGE | Solanum habrochaites |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.76 | 3-OMT | - |
Solanum habrochaites |
2.1.1.76 | flavonol 3-O-methyltransferase | - |
Solanum habrochaites |
2.1.1.76 | MOMT3 | - |
Solanum habrochaites |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.76 | 0.39 | - |
7-O-methylmyricetin | in 50 mM Tris-HCl (pH 7.5), at 22°C | Solanum habrochaites | |
2.1.1.76 | 0.82 | - |
myricetin | in 50 mM Tris-HCl (pH 7.5), at 22°C | Solanum habrochaites | |
2.1.1.76 | 1.66 | - |
3'-O-methylmyricetin | in 50 mM Tris-HCl (pH 7.5), at 22°C | Solanum habrochaites | |
2.1.1.76 | 2.94 | - |
S-adenosyl-L-methionine | in 50 mM Tris-HCl (pH 7.5), at 22°C | Solanum habrochaites |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.76 | 50 | - |
7-O-methylmyricetin | in 50 mM Tris-HCl (pH 7.5), at 22°C | Solanum habrochaites | |
2.1.1.76 | 290 | - |
S-adenosyl-L-methionine | in 50 mM Tris-HCl (pH 7.5), at 22°C | Solanum habrochaites | |
2.1.1.76 | 930 | - |
3'-O-methylmyricetin | in 50 mM Tris-HCl (pH 7.5), at 22°C | Solanum habrochaites | |
2.1.1.76 | 1490 | - |
myricetin | in 50 mM Tris-HCl (pH 7.5), at 22°C | Solanum habrochaites |