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Literature summary extracted from

  • Schmidt, A.; Li, C.; Daniel Jones, A.; Pichersky, E.
    Characterization of a flavonol 3-O-methyltransferase in the trichomes of the wild tomato species Solanum habrochaites (2012), Planta, 236, 839-849.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.76 expressed in Escherichia coli Solanum habrochaites

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.76 3'-O-methylmyricetin i.e. laricitrin, inhibitory at substrate concentrations above 0.025 mM Solanum habrochaites

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.76 0.00055
-
 myricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
2.1.1.76 0.00207
-
 3'-O-methylmyricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
2.1.1.76 0.00998
-
 7-O-methylmyricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
2.1.1.76 0.01086
-
 S-adenosyl-L-methionine in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.76 40000
-
 x * 40000, SDS-PAGE Solanum habrochaites

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.76 additional information Solanum habrochaites the enzyme does not methylate 3',4',5'-O-trimethylmyricetin, 3,7,3',4'-O-methylquercetin, 3-methylquercetin, and 3,7,4'-O-trimethylkaempferol. The enzyme transfers a methyl group only to the 3-position hydroxyl ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.76 Solanum habrochaites
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.76 MonoQ column chromatography Solanum habrochaites

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.76 trichome glandular trichome Solanum habrochaites
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.76 additional information the enzyme does not methylate 3',4',5'-O-trimethylmyricetin, 3,7,3',4'-O-methylquercetin, 3-methylquercetin, and 3,7,4'-O-trimethylkaempferol. The enzyme transfers a methyl group only to the 3-position hydroxyl Solanum habrochaites ?
-
 ?
2.1.1.76 S-adenosyl-L-methionine + 3',5'-O-dimethylmyricetin 18% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,3',5'-O-trimethylmyricetin
-
 ?
2.1.1.76 S-adenosyl-L-methionine + 3'-O-methylmyricetin 64% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,3'-O-dimethylmyricetin
-
 ?
2.1.1.76 S-adenosyl-L-methionine + 3'-O-methylmyricetin i.e. laricitrin, 43% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,3'-O-dimethylmyricetin
-
 ?
2.1.1.76 S-adenosyl-L-methionine + 3-O-methylquercetin 3% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.76 S-adenosyl-L-methionine + 4'-O-methylkaempferol 11% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,4'-O-dimethylkaempferol
-
 ?
2.1.1.76 S-adenosyl-L-methionine + 7-O-methylmyricetin 104% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3,7-O-dimethylquercetin
-
 ?
2.1.1.76 S-adenosyl-L-methionine + kaempferol 53% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3-O-methylkaempferol
-
 ?
2.1.1.76 S-adenosyl-L-methionine + myricetin 100% activity Solanum habrochaites S-adenosyl-L-homocysteine + 3-O-methylmyricetin
-
 ?
2.1.1.76 S-adenosyl-L-methionine + quercetin 121% activity compared to myricetin Solanum habrochaites S-adenosyl-L-homocysteine + 3-O-methylquercetin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.76 ? x * 40000, SDS-PAGE Solanum habrochaites

Synonyms

EC Number Synonyms Comment Organism
2.1.1.76 3-OMT
-
 Solanum habrochaites
2.1.1.76 flavonol 3-O-methyltransferase
-
 Solanum habrochaites
2.1.1.76 MOMT3
-
 Solanum habrochaites

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.76 0.39
-
 7-O-methylmyricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
2.1.1.76 0.82
-
 myricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
2.1.1.76 1.66
-
 3'-O-methylmyricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
2.1.1.76 2.94
-
 S-adenosyl-L-methionine in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.76 50
-
 7-O-methylmyricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
2.1.1.76 290
-
 S-adenosyl-L-methionine in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
2.1.1.76 930
-
 3'-O-methylmyricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites
2.1.1.76 1490
-
 myricetin in 50 mM Tris-HCl (pH 7.5), at 22°C Solanum habrochaites