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Literature summary extracted from

  • Sue, M.; Nakamura, C.; Miyamoto, T.; Yajima, S.
    Active-site architecture of benzoxazinone-glucoside beta-D-glucosidases in Triticeae (2011), Plant Sci., 180, 268-275.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.182 expressed in Escherichia coli as His-tagged fusion protein Triticum aestivum
3.2.1.182 expressed in Escherichia coli as His-tagged fusion protein Secale cereale

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.182 crystal structures of an inactive mutant of the wheat glucosidase complexed with the natural substrate DIMBOA-Glc, wheat and rye glucosidases complexed with an aglycone DIMBOA, and wheat and rye glucosidases complexed with an inhibitor 2-fluoro-2- deoxy-beta-D-glucose are analyzed Triticum aestivum
3.2.1.182 crystal structures of an inactive mutant of the wheat glucosidase complexed with the natural substrate DIMBOA-Glc, wheat and rye glucosidases complexed with an aglycone DIMBOA, and wheat and rye glucosidases complexed with an inhibitor 2-fluoro-2- deoxy-beta-D-glucose are analyzed Secale cereale

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.182 E191A crystals of the enzymatically inactive mutant E191A are soaked in a solution containing DIMBOA-Glc as substrate and 3.5M sodium formate as cryoprotectant, the structure is solved at a resolution of 2.2 A and the DIMBOA-Glc molecule is clearly defined. In the structure, the aglycone moiety is shown to be stabilized by interaction with the aromatic ring of W379 and by a hydrogen bond with T194 Triticum aestivum
3.2.1.182 E462A inactive mutant, E462 plays a crucial role in hydrolysis of the substrate Triticum aestivum
3.2.1.182 G464S/S465L mutation reduces the reaction efficiency toward DIBOA-Glc to 36% of wild type, whereas it enhances efficiency toward DIMBOA-Glc to 400% of wild type Secale cereale

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.182 2-fluoro-2-deoxy-beta-D-glucose
-
Secale cereale
3.2.1.182 2-fluoro-2-deoxy-beta-D-glucose
-
Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.182 Secale cereale Q9FYS3
-
-
3.2.1.182 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.182 using HisTrap HP column and and Superdex 200 HR Triticum aestivum
3.2.1.182 using HisTrap HP column and and Superdex 200 HR Secale cereale

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.182 4-hydroxy-3-oxo-3,4,dihydro-2H-1,4,benzoxazin-2-yl beta-D-gluco-pyranoside + H2O
-
Secale cereale 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + beta-D-glucose
-
?
3.2.1.182 DIBOA-beta-D-glucoside + H2O TaGlu1b hydrolyzes DIMBOA-Glc much better than DIBOA-Glc Triticum aestivum DIBOA + beta-D-glucose
-
?
3.2.1.182 DIBOA-glucoside + H2O ScGlu shows similar enzyme activity toward DIMBOA-Glc and DIBOA-Glc Secale cereale DIBOA + beta-D-glucose
-
?
3.2.1.182 DIMBOA-beta-D-glucoside + H2O TaGlu1b hydrolyzes DIMBOA-Glc much better than DIBOA-Glc Triticum aestivum DIMBOA + beta-D-glucose
-
?
3.2.1.182 DIMBOA-glucoside + H2O ScGlu shows similar enzyme activity toward DIMBOA-Glc and DIBOA-Glc Secale cereale DIMBOA + beta-D-glucose
-
?
3.2.1.182 esculin + H2O
-
Secale cereale esculetin + beta-D-glucose
-
?
3.2.1.182 genistin + H2O
-
Secale cereale ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.182 benzoxazinone-glucoside beta-D-glucosidase
-
Triticum aestivum
3.2.1.182 benzoxazinone-glucoside beta-D-glucosidase
-
Secale cereale
3.2.1.182 ScGlu
-
Secale cereale
3.2.1.182 TaGlu1
-
Triticum aestivum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.182 30
-
assay at Triticum aestivum
3.2.1.182 30
-
assay at Secale cereale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.182 5.5
-
assay at Triticum aestivum
3.2.1.182 5.5
-
assay at Secale cereale

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.182 120
-
DIMBOA-glucoside pH and temperature not specified in the publication Secale cereale
3.2.1.182 150
-
DIBOA-glucoside pH and temperature not specified in the publication Secale cereale
3.2.1.182 150
-
DIBOA-beta-D-glucoside pH and temperature not specified in the publication Triticum aestivum
3.2.1.182 4100
-
DIMBOA-beta-D-glucoside pH and temperature not specified in the publication Triticum aestivum