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Literature summary extracted from

  • van Erp, H.; Bates, P.D.; Burgal, J.; Shockey, J.; Browse, J.
    Castor phospholipid:diacylglycerol acyltransferase facilitates efficient metabolism of hydroxy fatty acids in transgenic Arabidopsis (2011), Plant Physiol., 155, 683-693.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.158 synthesis the enzyme is useful for production of hydroxylated fatty acids and furtheron polyesters, biodiesel, and lubricants in transgenic plants expressing PDAT thereby avoiding the toxicity of castor bean seeds Ricinus communis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.158 isozyme PDAT2 and PDAT1A, cloning from cDNA library, expression in Arabidopsis thaliana, coexpression of PDAT1A with Ricinus communis hydroxylated fatty acid hydroxylase, RcFAH, in Arabidopsis thaliana causes a reduction of fatty acids by 73% compared to wild-type plants, isozyme PDAT2 is also functional but less active than isozyme PDAT1A, method optimization, phenotypes, overview Ricinus communis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.158 microsome
-
 Ricinus communis
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol Ricinus communis
-
 lysophospholipid + triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.158 Ricinus communis
-
 three genes encoding isozymes PDAT1A and PDAT2, and PDAT1B
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.158 endosperm both PDAT1A and PDAT2 are expressed in the developing endosperm, with PDAT2 showing double the transcript levels of PDAT1A. No expression of RcPDAT1B in castor endosperm Ricinus communis
-
2.3.1.158 seed
-
 Ricinus communis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol
-
 Ricinus communis lysophospholipid + triacylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.158 PDAT
-
 Ricinus communis
2.3.1.158 PDAT1A
-
 Ricinus communis