Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Raharjo, T.; Chang, W.; Verberne, M.; Peltenburg-Looman, A.; Linthorst, H.; Verpoorte, R.
    Cloning and over-expression of a cDNA encoding a polyketide synthase from Cannabis sativa (2004), Plant Physiol. Biochem., 42, 291-297.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.206 gene pks, cDNA and amino acid sequence determination and analysis, overexpression of His-tagged PKS in Escherichia coli strain M15 Cannabis sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.206 42700
-
 x * 42700, about, sequence calculation, x * 45000, SDS-PAGE Cannabis sativa
2.3.1.206 45000
-
 x * 42700, about, sequence calculation, x * 45000, SDS-PAGE Cannabis sativa

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.206 Cannabis sativa
-
 gene pks
-
2.3.1.206 Cannabis sativa Four-way
-
 gene pks
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.206 leaf young Cannabis sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.206 3 malonyl-CoA + hexanoyl-CoA formation of a C12-polyketide, which is formed to olivetol and its carboxylated derivative olivetolic acid by an aldol reaction thrugh stilbene carboxylate synthase-like activity, overview Cannabis sativa ?
-
 ?
2.3.1.206 3 malonyl-CoA + hexanoyl-CoA formation of a C12-polyketide, which is formed to olivetol and its carboxylated derivative olivetolic acid by an aldol reaction thrugh stilbene carboxylate synthase-like activity, overview Cannabis sativa Four-way ?
-
 ?
2.3.1.206 additional information usage of 4-coumaroyl-CoA and malonyl-CoA as substrates, resulting product is naringenin, not resveratrol. The enzyme might show a broad specificity of substrate including aliphatic substrates such as isovaleryl-CoA, isobutyryl-CoA, and n-hexanoyl-CoA Cannabis sativa ?
-
 ?
2.3.1.206 additional information usage of 4-coumaroyl-CoA and malonyl-CoA as substrates, resulting product is naringenin, not resveratrol. The enzyme might show a broad specificity of substrate including aliphatic substrates such as isovaleryl-CoA, isobutyryl-CoA, and n-hexanoyl-CoA Cannabis sativa Four-way ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.206 ? x * 42700, about, sequence calculation, x * 45000, SDS-PAGE Cannabis sativa

Synonyms

EC Number Synonyms Comment Organism
2.3.1.206 polyketide synthase
-
 Cannabis sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.206 30
-
 assay at Cannabis sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.206 6.8
-
 assay at Cannabis sativa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.206 Cannabis sativa sequence calculation
-
 6.04

General Information

EC Number General Information Comment Organism
2.3.1.206 physiological function polyketide synthases play an important role in the biosynthesis of secondary metabolites such as resveratrol, a candidate for cancer chemoprevention and naringenin, the precursor for flavonoids. Olivetolic acid is also be expected to be synthesized by a PKS Cannabis sativa