EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.1.160 | food industry | producing low-caffeine tea through post-transcriptional silencing of caffeine synthase mRNA | Camellia sinensis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.160 | semiquantitative-PCR for caffeine synthase transcript expression analysis | Camellia sinensis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.160 | additional information | isolation of a partial fragment of caffeine synthase from cv. Kangra jat and usage for design an RNAi construct, pFGC1008-CS, detection of siRNAs specific to caffeine synthase in transgenic plants. Transformation of somatic embryos with the developed construct using a biolistic method. Transformed somatic embryos show reduction in the levels of caffeine synthase transcript expression as well as in caffeine content. Transgenic plants generated from the transformed somatic embryos show a significant suppression of caffeine synthase transcript expression and also a reduction of 44-61% in caffeine and 46-67% in theobromine contents as compared to the controls | Camellia sinensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.160 | S-adenosyl-L-methionine + 3,7-dimethylxanthine | Camellia sinensis | - |
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
2.1.1.160 | S-adenosyl-L-methionine + 7-methylxanthine | Camellia sinensis | - |
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.160 | Camellia sinensis | - |
cv. Kangra jat | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.160 | leaf | - |
Camellia sinensis | - |
2.1.1.160 | seedling | - |
Camellia sinensis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.160 | S-adenosyl-L-methionine + 3,7-dimethylxanthine | - |
Camellia sinensis | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
2.1.1.160 | S-adenosyl-L-methionine + 7-methylxanthine | - |
Camellia sinensis | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.160 | 3, 7-dimethylxanthine methyltransferase | - |
Camellia sinensis |
2.1.1.160 | 7-N-methylxanthine methyltransferase | - |
Camellia sinensis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.160 | S-adenosyl-L-methionine | - |
Camellia sinensis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.160 | malfunction | post-transcriptional silencing of caffeine synthase mRNA leads to reduced caffeine content in tea leaves | Camellia sinensis |
2.1.1.160 | metabolism | caffeine synthase is a bifunctional enzyme that catalyzes the last two steps of caffeine biosynthesis in tea as 7-N-methylxanthine methyltransferase and 3,7-dimethylxanthine methyltransferase | Camellia sinensis |