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Literature summary extracted from

  • He, W.; Brumos, J.; Li, H.; Ji, Y.; Ke, M.; Gong, X.; Zeng, Q.; Li, W.; Zhang, X.; An, F.; Wen, X.; Li, P.; Chu, J.; Sun, X.; Yan, C.; Yan, N.; Xie, D.Y.; Raikhel, N.; Yang, Z.; Stepanova, A.N.; Alonso, J.M.; Guo, H.
    A small-molecule screen identifies L-kynurenine as a competitive inhibitor of TAA1/TAR activity in ethylene-directed auxin biosynthesis and root growth in Arabidopsis (2011), Plant Cell, 23, 3944-3960.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.27 expressed in Escherichia coli Arabidopsis thaliana
2.6.1.99 expression of GST-TAA1 in Escherichia coli strain BL21 Star (DE3) pLysS Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.27 L-kynurenine competitively inhibits TAA1/TAR activity, and Kyn treatment mimicks the loss of TAA1/TAR functions; competitively inhibits TAA1/TAR activity, and Kyn treatment mimicks the loss of TAA1/TAR functions Arabidopsis thaliana
2.6.1.99 L-kynurenine an alternate substrate, competitively inhibits TAA1/TAR activity, and Kyn treatment mimicks the loss of TAA1/TAR functions. L-kynurenine application represses activity and nuclear accumulation of the EIN3 transcription factor in roots. L-kynurenine effectively and selectively binds to the substrate pocket of TAA1/TAR proteins but not to those of other families of aminotransferases, computational docking and molecular modeling, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.99 L-tryptophan + pyruvate Arabidopsis thaliana
-
 indole-3-pyruvate + L-alanine
-
 ?
2.6.1.99 L-tryptophan + pyruvate Arabidopsis thaliana Col-0
-
 indole-3-pyruvate + L-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.27 Arabidopsis thaliana Q9LR29
-
-
2.6.1.27 Arabidopsis thaliana Q9S7N2
-
-
2.6.1.99 Arabidopsis thaliana Q9S7N2
-
-
2.6.1.99 Arabidopsis thaliana Col-0 Q9S7N2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.99 root
-
 Arabidopsis thaliana
-
2.6.1.99 seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.27 additional information TAA1 can also use Kyn as a substrate in vitro to produce kynurenic acid Arabidopsis thaliana ?
-
 ?
2.6.1.27 additional information TAA1 can also use Kyn as a substrate in vitro to produce kynurenic acid Arabidopsis thaliana Col-0 ?
-
 ?
2.6.1.99 L-tryptophan + pyruvate
-
 Arabidopsis thaliana indole-3-pyruvate + L-alanine
-
 ?
2.6.1.99 L-tryptophan + pyruvate
-
 Arabidopsis thaliana Col-0 indole-3-pyruvate + L-alanine
-
 ?
2.6.1.99 additional information L-kynurenine is an alternate substrate competing with L-tryptophan Arabidopsis thaliana ?
-
 ?
2.6.1.99 additional information L-kynurenine is an alternate substrate competing with L-tryptophan Arabidopsis thaliana Col-0 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.27 TAA1
-
 Arabidopsis thaliana
2.6.1.27 TAR1
-
 Arabidopsis thaliana
2.6.1.99 TAA1
-
 Arabidopsis thaliana
2.6.1.99 tryptophan aminotransferase of Arabidopsis 1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.27 55
-
 assay at Arabidopsis thaliana
2.6.1.99 55
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.99 pyridoxal 5'-phosphate
-
 Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.27 0.01152
-
 L-kynurenine pH and temperature not specified in the publication Arabidopsis thaliana
2.6.1.99 0.01152
-
 L-kynurenine inhibition of TAA1, competitive versus L-Tryp, pH not specificied in the publication, 55°C Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.6.1.99 malfunction L-kynurenine, being an alternate substrate, competitively inhibits TAA1/TAR activity, and Kyn treatment mimicks the loss of TAA1/TAR functions Arabidopsis thaliana
2.6.1.99 metabolism tryptophan aminotransferase of Arabidopsis 1 and tryptopan aminotransferase related, i.e. TAA1 and TAR, are the key enzymes in the indole-3-pyruvic acid pathway of auxin biosynthesis Arabidopsis thaliana