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Literature summary extracted from

  • Wang, H.; Nagegowda, D.A.; Rawat, R.; Bouvier-Nave, P.; Guo, D.; Bach, T.J.; Chye, M.L.
    Overexpression of Brassica juncea wild-type and mutant HMG-CoA synthase 1 in Arabidopsis up-regulates genes in sterol biosynthesis and enhances sterol production and stress tolerance (2012), Plant Biotechnol. J., 10, 31-42.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.10 expressed in Arabidopsis thaliana Brassica juncea

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.10 H188N the mutant lacks substrate inhibition by acetoacetyl-CoA and shows 8fold decreased enzyme activity Brassica juncea
2.3.3.10 H188N/S359A the double mutant displays a 10fold increased enzyme activity and lacks inhibition by acetoacetyl-CoA Brassica juncea
2.3.3.10 S359A the mutant demonstrates 10fold higher activity as the wild type enzyme Brassica juncea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 acetoacetyl-CoA substrate inhibition Brassica juncea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.10 cytosol
-
 Brassica juncea 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Brassica juncea
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Brassica juncea (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.10 3-hydroxy-3-methylglutaryl-CoA synthase
-
 Brassica juncea
2.3.3.10 HMG-CoA synthase 1
-
 Brassica juncea
2.3.3.10 HMGS
-
 Brassica juncea

General Information

EC Number General Information Comment Organism
2.3.3.10 metabolism 3-hydroxy-3-methylglutaryl-CoA synthase is the second enzyme in the cytosolic mevalonate pathway Brassica juncea
2.3.3.10 physiological function enzyme overexpression up-regulates 3-hydroxy-3-methylglutaryl-CoA reductase, sterol methyltransferase 2, delta-24 sterol reductase, C-22 sterol desaturase, and brassinosteroid-6-oxidase 2, leading to enhanced sterol content and stress tolerance in Arabidopsis. Enzyme overexpressing Arabidopsis seeds germinate approximately 1 day earlier than wild type seeds. Enzyme overexpressing Arabidopsis seedlings show enhanced resistance to Botrytis cinerea, and detached leaves are delayed in H2O2-induced cell death Brassica juncea