Literature summary extracted from

  • Nikus, J.; Esen, A.; Jonsson, L.
    Cloning of a plastidic rye (Secale cereale) beta-glucosidase cDNA and its expression in Escherichia coli (2003), Physiol. Plant., 118, 337-345.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.182 expressed in Escherichia coli Secale cereale

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.182 1.3
-
4-nitrophenyl beta-D-galactoside pH 5.8, 37°C, native protein Secale cereale
3.2.1.182 1.3
-
2-Nitrophenyl beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
3.2.1.182 0.8
-
4-nitrophenyl beta-D-fucoside pH 5.8, 37°C, native protein Secale cereale
3.2.1.182 1.4
-
2-Nitrophenyl beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
3.2.1.182 1.4
-
DIMBOA beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
3.2.1.182 2.4
-
4-nitrophenyl beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
3.2.1.182 2.4
-
4-nitrophenyl beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
3.2.1.182 1.5
-
4-nitrophenyl beta-D-galactoside pH 5.8, 37°C, recombinant protein Secale cereale
3.2.1.182 3.3
-
DIBOA beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
3.2.1.182 2.1
-
DIMBOA beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
3.2.1.182 2.8
-
DIBOA beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
3.2.1.182 1.1
-
4-nitrophenyl beta-D-fucoside pH 5.8, 37°C, recombinant protein Secale cereale

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.182 chloroplast putatively localised in chloroplasts Secale cereale
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.182 67000
-
SDS-PAGE Secale cereale

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.182 Secale cereale
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.182 coleoptile highest expression Secale cereale
-
3.2.1.182 mesocotyl highest expression Secale cereale
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.182 4-nitrophenyl beta-D-fucoside + H2O
-
Secale cereale 4-nitrophenol + beta-D-fucose
-
?
3.2.1.182 4-nitrophenyl beta-D-galactoside + H2O
-
Secale cereale 4-nitrophenol + beta-D-galactose
-
?
3.2.1.182 2-nitrophenyl beta-D-glucoside + H2O
-
Secale cereale 2-nitrophenol + beta-D-glucose
-
?
3.2.1.182 4-nitrophenyl beta-D-glucoside + H2O
-
Secale cereale 4-nitrophenol + beta-D-glucose
-
?
3.2.1.182 DIBOA beta-D-glucoside + H2O
-
Secale cereale DIBOA + beta-D-glucose
-
?
3.2.1.182 DIMBOA beta-D-glucoside + H2O
-
Secale cereale DIMBOA + beta-D-glucose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.182 beta-glucosidase
-
Secale cereale

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.182 37
-
assay at Secale cereale

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.182 3.3
-
4-nitrophenyl beta-D-galactoside pH 5.8, 37°C, recombinant protein Secale cereale
3.2.1.182 3.5
-
4-nitrophenyl beta-D-galactoside pH 5.8, 37°C, native protein Secale cereale
3.2.1.182 169
-
DIBOA beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
3.2.1.182 52
-
4-nitrophenyl beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
3.2.1.182 119
-
DIMBOA beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
3.2.1.182 159
-
2-Nitrophenyl beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
3.2.1.182 172
-
DIBOA beta-D-glucoside pH 5.8, 37°C, native protein Secale cereale
3.2.1.182 86.5
-
4-nitrophenyl beta-D-fucoside pH 5.8, 37°C, recombinant protein Secale cereale
3.2.1.182 72.8
-
4-nitrophenyl beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
3.2.1.182 97.4
-
DIMBOA beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
3.2.1.182 204.2
-
2-Nitrophenyl beta-D-glucoside pH 5.8, 37°C, recombinant protein Secale cereale
3.2.1.182 66.8
-
4-nitrophenyl beta-D-fucoside pH 5.8, 37°C, native protein Secale cereale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.182 5.8
-
assay at Secale cereale