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Literature summary extracted from

  • Poulin, M.B.; Zhou, R.; Lowary, T.L.
    Synthetic UDP-galactofuranose analogs reveal critical enzyme-substrate interactions in GlfT2-catalyzed mycobacterial galactan assembly (2012), Org. Biomol. Chem., 10, 4074-4087.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.288 additional information not inhibited by UDP-2''-O-methyl-alpha-D-galactofuranose Mycobacterium tuberculosis
2.4.1.288 UDP-3''-deoxy-alpha-D-xylo-hexofuranose 68% inhibition at 1.25 mM Mycobacterium tuberculosis
2.4.1.288 UDP-5''-O-methyl-alpha-D-galactofuranose 15% inhibition at 1.25 mM Mycobacterium tuberculosis
2.4.1.288 UDP-5-deoxy-alpha-D-galactofuranose 10% inhibition at 1.25 mM Mycobacterium tuberculosis
2.4.1.288 UDP-6''-O-methyl-alpha-D-galactofuranose 26% inhibition at 1.25 mM Mycobacterium tuberculosis
2.4.1.288 UDP-alpha-D-arabinofuranose less than 1% inhibition at 1.25 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.288 0.25
-
 UDP-alpha-D-galactofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.5
-
 UDP-3-deoxy-alpha-D-threo-hexofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.9
-
 UDP-5-deoxy-alpha-D-galactofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.9
-
 UDP-alpha-D-arabinofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 1
-
 UDP-6-deoxy-alpha-D-galactofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 1
-
 UDP-alpha-D-galactofuranose using beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 1.9
-
 dTDP-alpha-D-galactofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.288 Mycobacterium tuberculosis
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.288 0.0008
-
 using UDP-5-deoxy-alpha-D-galactofuranose and beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.004
-
 using UDP-2''-O-methyl-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.006
-
 using UDP-5''-O-methyl-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.007
-
 using UDP-6''-O-methyl-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.01
-
 using UDP-3-deoxy-alpha-D-threo-hexofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.01
-
 using UDP-alpha-D-arabinofuranose and beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.012
-
 using UDP-6-deoxy-alpha-D-galactofuranose and beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.1
-
 using UDP-5-deoxy-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.14
-
 using UDP-alpha-D-arabinofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.14
-
 using UDP-alpha-D-galactofuranose and beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.35
-
 using dTDP-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.9
-
 using UDP-6-deoxy-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 1.6
-
 using UDP-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.288 dTDP-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-2''-O-methyl-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-3''-deoxy-alpha-D-xylo-hexofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-3-deoxy-alpha-D-threo-hexofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-5''-O-methyl-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-5-deoxy-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-5-deoxy-alpha-D-galactofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-6''-O-methyl-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-6-deoxy-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-6-deoxy-alpha-D-galactofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-alpha-D-arabinofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-alpha-D-arabinofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl best donor-acceptor combination, dual recognition of both the terminal C-5 and C-6 hydroxyl groups of the acceptor substrate are required for GlfT2 activity Mycobacterium tuberculosis UDP + ?
-
 ?
2.4.1.288 UDP-alpha-D-galactofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl
-
 Mycobacterium tuberculosis UDP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.288 GlfT2
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.288 0.02
-
 UDP-3-deoxy-alpha-D-threo-hexofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.17
-
 UDP-5-deoxy-alpha-D-galactofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.25
-
 UDP-alpha-D-arabinofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.33
-
 UDP-alpha-D-galactofuranose using beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 1.32
-
 UDP-6-deoxy-alpha-D-galactofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 1.62
-
 dTDP-alpha-D-galactofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 2.18
-
 UDP-alpha-D-galactofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.288 0.12
-
 UDP-3''-deoxy-alpha-D-xylo-hexofuranose in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.288 0.04
-
 UDP-3-deoxy-alpha-D-threo-hexofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.2
-
 UDP-5-deoxy-alpha-D-galactofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.25
-
 UDP-alpha-D-arabinofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.33
-
 dTDP-alpha-D-galactofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 0.33
-
 UDP-alpha-D-galactofuranose using beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 1.17
-
 UDP-6-deoxy-alpha-D-galactofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis
2.4.1.288 8.3
-
 UDP-alpha-D-galactofuranose using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C Mycobacterium tuberculosis