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Literature summary extracted from

  • Iyama, T.; Abolhassani, N.; Tsuchimoto, D.; Nonaka, M.; Nakabeppu, Y.
    NUDT16 is a (deoxy)inosine diphosphatase, and its deficiency induces accumulation of single-strand breaks in nuclear DNA and growth arrest (2010), Nucleic Acids Res., 38, 4834-4843.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.64 expression in Escherichia coli Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.64 0.062
-
 IDP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.088
-
 dIDP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.32
-
 dGDP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.33
-
 GDP pH 7.5, 37°C Homo sapiens
3.6.1.64 15.7
-
 XDP pH 7.5, 37°C Homo sapiens
3.6.1.64 22.1
-
 ITP pH 7.5, 37°C Homo sapiens
3.6.1.64 24.1
-
 dITP pH 7.5, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.64 nucleolus
-
 Homo sapiens 5730
-
3.6.1.64 nucleolus
-
 Mus musculus 5730
-
3.6.1.64 nucleus
-
 Homo sapiens 5634
-
3.6.1.64 nucleus
-
 Mus musculus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.64 Mg2+ maximum activity at 1 mM Homo sapiens
3.6.1.64 additional information divalent cation absolutely required. No IDP hydrolysis in the presence of 5mM Mn2+, Co2+ or Ca2+ Homo sapiens
3.6.1.64 Zn2+ may partly substitue for Mn2+ Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.64 Homo sapiens
-
-
-
3.6.1.64 Homo sapiens Q96DE0 bifunctional U8 snoRNA-decapping enzyme and IDP phosphatase
-
3.6.1.64 Mus musculus Q6P3D0 bifunctional U8 snoRNA-decapping enzyme and IDP phosphatase
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.6.1.64 dIDP + H2O = dIMP + phosphate (2) Homo sapiens
a
3.6.1.64 IDP + H2O = IMP + phosphate (1) Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.64 kidney and lung, highest expression Homo sapiens
-
3.6.1.64 lung and kidney, highest expression Homo sapiens
-
3.6.1.64 additional information expression in all tissues examined Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.64 dGDP + H2O
-
 Homo sapiens dGMP + phosphate
-
 ?
3.6.1.64 dIDP + H2O
-
 Homo sapiens dIMP + phosphate
-
 ?
3.6.1.64 dITP + H2O
-
 Mus musculus dIMP + diphosphate
-
 ?
3.6.1.64 dITP + H2O
-
 Homo sapiens dIMP + diphosphate hydrolysis of ITP generates IMP but not phosphate ?
3.6.1.64 GDP + H2O
-
 Homo sapiens GMP + phosphate
-
 ?
3.6.1.64 IDP + H2O
-
 Homo sapiens IMP + phosphate
-
 ?
3.6.1.64 IDP + H2O
-
 Mus musculus IMP + phosphate
-
 ?
3.6.1.64 ITP + H2O
-
 Homo sapiens IDP + phosphate
-
 ?
3.6.1.64 XDP + H2O
-
 Homo sapiens XMP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.64 (deoxy)inosine diphosphatase
-
 Homo sapiens
3.6.1.64 NUD16
-
 Homo sapiens
3.6.1.64 NUD16
-
 Mus musculus
3.6.1.64 Nudt16
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.64 60
-
 temperature-dependent increase in IDP hydrolyzing activity up to 60°C Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.64 0.008
-
 dGDP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.009
-
 GDP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.016
-
 IDP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.016
-
 dIDP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.043
-
 XDP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.051
-
 ITP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.053
-
 dITP pH 7.5, 37°C Homo sapiens

General Information

EC Number General Information Comment Organism
3.6.1.64 physiological function knockdown in HeLa MR cells causes cell cycle arrest in S-phase, reduces cell proliferation, and leads to increased accumulation of single-strand breaks in nuclear DNA as well as increased levels of inosine in RNA Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.64 0.0022
-
 dITP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.0023
-
 ITP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.0027
-
 XDP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.0257
-
 dGDP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.0261
-
 GDP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.183
-
 dIDP pH 7.5, 37°C Homo sapiens
3.6.1.64 0.251
-
 IDP pH 7.5, 37°C Homo sapiens