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Literature summary extracted from

  • Ohtani, N.; Yanagawa, H.; Tomita, M.; Itaya, M.
    Cleavage of double-stranded RNA by RNase HI from a thermoacidophilic archaeon, Sulfolobus tokodaii 7 (2004), Nucleic Acids Res., 32, 5809-5819.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.4 D125N mutation results in loss of dsRNase activity but not RNase H activity Sulfurisphaera tokodaii
3.1.26.4 D76N no RNase H activity is detected Sulfurisphaera tokodaii
3.1.26.4 D7N enzyme loses RNase H activity and dsRNase activity in the presence of MnCl2 Sulfurisphaera tokodaii
3.1.26.4 E52Q no RNase H activity is detected Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 Co2+ the enzyme cleaves an RNA strand of the 12-bp RNA/DNA hybrid at multiple sites only in the presence of Mn2+, Mg2+, Co2+ or Ni2+, but not in the presence of Cu2+, Ca2+ or Zn2+, or in the absence of divalent metal ions. The enzyme cleaves an RNA/RNA duplex in the presence of Mn2+ or Co2+ Sulfurisphaera tokodaii
3.1.26.4 Mg2+ the enzyme cleaves an RNA strand of the 12-bp RNA/DNA hybrid at multiple sites only in the presence of Mn2+, Mg2+, Co2+ or Ni2+, but not in the presence of Cu2+, Ca2+ or Zn2+, or in the absence of divalent metal ions Sulfurisphaera tokodaii
3.1.26.4 Mn2+ the enzyme cleaves an RNA strand of the 12-bp RNA/DNA hybrid at multiple sites only in the presence of Mn2+, Mg2+, Co2+ or Ni2+, but not in the presence of Cu2+, Ca2+ or Zn2+, or in the absence of divalent metal ions. The enzyme cleaves an RNA/RNA duplex in the presence of Mn2+ or Co2+ Sulfurisphaera tokodaii
3.1.26.4 Ni2+ the enzyme cleaves an RNA strand of the 12-bp RNA/DNA hybrid at multiple sites only in the presence of Mn2+, Mg2+, Co2+ or Ni2+, but not in the presence of Cu2+, Ca2+ or Zn2+, or in the absence of divalent metal ions Sulfurisphaera tokodaii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.4 13000
-
 gel filtration Sulfurisphaera tokodaii
3.1.26.4 16812
-
 1 * 16812, calculated from sequence Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Sulfurisphaera tokodaii F9VN79
-
-
3.1.26.4 Sulfurisphaera tokodaii 7 F9VN79
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4
-
 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 RNA + H2O the enzyme cleaves the RNA strand of an RNA/DNA hybrid or an RNA/RNA duplex in the presence of Mn2+ or Co2+ Sulfurisphaera tokodaii ?
-
 ?
3.1.26.4 RNA + H2O the enzyme cleaves the RNA strand of an RNA/DNA hybrid or an RNA/RNA duplex in the presence of Mn2+ or Co2+ Sulfurisphaera tokodaii 7 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.26.4 monomer 1 * 16812, calculated from sequence Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
3.1.26.4 protein ST0753
-
 Sulfurisphaera tokodaii
3.1.26.4 RNase HI
-
 Sulfurisphaera tokodaii
3.1.26.4 type 1 RNase H
-
 Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.4 8.5
-
 the standard cleavage reaction is performed at a pH of 8.5 in the presence of 1 mM MgCl2 or MnCl2, because at a higher pH range, the solubility of divalent metal ions decreases and RNA/DNA substrates might become destabilized Sulfurisphaera tokodaii
3.1.26.4 10
-
 the cleavage activity increases exponentially as the pH increases from 6 to 10 Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.26.4 6 10 the cleavage activity increases exponentially as the pH increases from 6 to 10 Sulfurisphaera tokodaii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.26.4 Sulfurisphaera tokodaii calculated from sequence
-
 9.5