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Literature summary extracted from

  • Desogus, G.; Onesti, S.; Brick, P.; Rossi, M.; Pisani, F.M.
    Identification and characterization of a DNA primase from the hyperthermophilic archaeon Methanococcus jannaschii (1999), Nucleic Acids Res., 27, 4444-4450.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.B16 overexpression in Escherichia coli Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.B16 K+ activity requires divalent cations such Mg2+, Mn2+ or Zn2+, and is additionally stimulated by the monovalent cation K+ Methanocaldococcus jannaschii
2.7.7.B16 Mg2+ activity requires divalent cations such Mg2+, Mn2+ or Zn2+, and is additionally stimulated by the monovalent cation K+ Methanocaldococcus jannaschii
2.7.7.B16 Mn2+ activity requires divalent cations such Mg2+, Mn2+ or Zn2+, and is additionally stimulated by the monovalent cation K+ Methanocaldococcus jannaschii
2.7.7.B16 Zn2+ activity requires divalent cations such Mg2+, Mn2+ or Zn2+, and is additionally stimulated by the monovalent cation K+ Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.B16 41800
-
x * 41800, calculated from sequence Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B16 Methanocaldococcus jannaschii Q58249
-
-
2.7.7.B16 Methanocaldococcus jannaschii JAL-1 Q58249
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.B16
-
Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.B16 NTP + n NTP synthesise oligoribonucleotides on various pyrimidine single-stranded DNA templates poly(dT) and poly(dC). The enzyme is almost completely inactive on a poly(dA) synthetic template Methanocaldococcus jannaschii N(pN)n + n diphosphate
-
?
2.7.7.B16 NTP + n NTP synthesise oligoribonucleotides on various pyrimidine single-stranded DNA templates poly(dT) and poly(dC). The enzyme is almost completely inactive on a poly(dA) synthetic template Methanocaldococcus jannaschii JAL-1 N(pN)n + n diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.B16 ? x * 41800, calculated from sequence Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.7.7.B16 Mjpri
-
Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.B16 60
-
1 h, enzyme is stable Methanocaldococcus jannaschii
2.7.7.B16 70
-
10 min, 20% loss of activity Methanocaldococcus jannaschii
2.7.7.B16 80
-
half-life: 15 min Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.B16 8
-
-
Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.B16 7.5 8.5 pH 7.5: about 40% of maximal activity, pH 8.5: about 70% of maximal activity Methanocaldococcus jannaschii