EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.B16 | overexpression in Escherichia coli | Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.B16 | K+ | activity requires divalent cations such Mg2+, Mn2+ or Zn2+, and is additionally stimulated by the monovalent cation K+ | Methanocaldococcus jannaschii | |
2.7.7.B16 | Mg2+ | activity requires divalent cations such Mg2+, Mn2+ or Zn2+, and is additionally stimulated by the monovalent cation K+ | Methanocaldococcus jannaschii | |
2.7.7.B16 | Mn2+ | activity requires divalent cations such Mg2+, Mn2+ or Zn2+, and is additionally stimulated by the monovalent cation K+ | Methanocaldococcus jannaschii | |
2.7.7.B16 | Zn2+ | activity requires divalent cations such Mg2+, Mn2+ or Zn2+, and is additionally stimulated by the monovalent cation K+ | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.B16 | 41800 | - |
x * 41800, calculated from sequence | Methanocaldococcus jannaschii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.B16 | Methanocaldococcus jannaschii | Q58249 | - |
- |
2.7.7.B16 | Methanocaldococcus jannaschii JAL-1 | Q58249 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.B16 | - |
Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.B16 | NTP + n NTP | synthesise oligoribonucleotides on various pyrimidine single-stranded DNA templates poly(dT) and poly(dC). The enzyme is almost completely inactive on a poly(dA) synthetic template | Methanocaldococcus jannaschii | N(pN)n + n diphosphate | - |
? | |
2.7.7.B16 | NTP + n NTP | synthesise oligoribonucleotides on various pyrimidine single-stranded DNA templates poly(dT) and poly(dC). The enzyme is almost completely inactive on a poly(dA) synthetic template | Methanocaldococcus jannaschii JAL-1 | N(pN)n + n diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.B16 | ? | x * 41800, calculated from sequence | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.B16 | Mjpri | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.B16 | 60 | - |
1 h, enzyme is stable | Methanocaldococcus jannaschii |
2.7.7.B16 | 70 | - |
10 min, 20% loss of activity | Methanocaldococcus jannaschii |
2.7.7.B16 | 80 | - |
half-life: 15 min | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.B16 | 8 | - |
- |
Methanocaldococcus jannaschii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.B16 | 7.5 | 8.5 | pH 7.5: about 40% of maximal activity, pH 8.5: about 70% of maximal activity | Methanocaldococcus jannaschii |