BRENDA - Enzyme Database show

The Leishmania nicotinamidase is essential for NAD+ production and parasite proliferation

Gazanion, E.; Garcia, D.; Silvestre, R.; Gerard, C.; Guichou, J.F.; Labesse, G.; Seveno, M.; Cordeiro-Da-Silva, A.; Ouaissi, A.; Sereno, D.; Vergnes, B.; Mol. Microbiol. 82, 21-38 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.19
expressed in Escherichia coli BL21(DE3) cells
Leishmania infantum
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.5.1.19
sitting-drop-based and sparse-matrix screening , using 0.7 M Na-citrate and 0.1 M HEPES (pH 7.5)
Leishmania infantum
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.19
C161A
inactive
Leishmania infantum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.19
cytosol
under physiological conditions, the enzyme displays a diffuse, mainly cytosolic localization
Leishmania infantum
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
22900
-
x * 22900, calculated from amino acid sequence
Leishmania infantum
3.5.1.19
24000
-
x * 24000, SDS-PAGE
Leishmania infantum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Leishmania infantum
-
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Leishmania infantum MHOM/MA/67/ITMAP-263
-
nicotinate + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Leishmania infantum
-
pyrazine-2-carboxylic acid + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Leishmania infantum MHOM/MA/67/ITMAP-263
-
pyrazine-2-carboxylic acid + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Leishmania infantum
-
-
-
3.5.1.19
Leishmania infantum MHOM/MA/67/ITMAP-263
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.19
Ni-NTA agarose column chromatography
Leishmania infantum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.19
promastigote
-
Leishmania infantum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
720499
Leishmania infantum
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
720499
Leishmania infantum MHOM/MA/67/ITMAP-263
nicotinate + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
720499
Leishmania infantum
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
720499
Leishmania infantum MHOM/MA/67/ITMAP-263
pyrazine-2-carboxylic acid + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.19
?
x * 22900, calculated from amino acid sequence; x * 24000, SDS-PAGE
Leishmania infantum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
expressed in Escherichia coli BL21(DE3) cells
Leishmania infantum
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.5.1.19
sitting-drop-based and sparse-matrix screening , using 0.7 M Na-citrate and 0.1 M HEPES (pH 7.5)
Leishmania infantum
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.19
C161A
inactive
Leishmania infantum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.19
cytosol
under physiological conditions, the enzyme displays a diffuse, mainly cytosolic localization
Leishmania infantum
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.19
22900
-
x * 22900, calculated from amino acid sequence
Leishmania infantum
3.5.1.19
24000
-
x * 24000, SDS-PAGE
Leishmania infantum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Leishmania infantum
-
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Leishmania infantum MHOM/MA/67/ITMAP-263
-
nicotinate + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Leishmania infantum
-
pyrazine-2-carboxylic acid + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Leishmania infantum MHOM/MA/67/ITMAP-263
-
pyrazine-2-carboxylic acid + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
Ni-NTA agarose column chromatography
Leishmania infantum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.19
promastigote
-
Leishmania infantum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
720499
Leishmania infantum
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
720499
Leishmania infantum MHOM/MA/67/ITMAP-263
nicotinate + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
720499
Leishmania infantum
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
720499
Leishmania infantum MHOM/MA/67/ITMAP-263
pyrazine-2-carboxylic acid + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.19
?
x * 22900, calculated from amino acid sequence; x * 24000, SDS-PAGE
Leishmania infantum
General Information
EC Number
General Information
Commentary
Organism
3.5.1.19
malfunction
the generation of nicotinamidase null mutants leads to a decrease in NAD+ content, associated with a metabolic shutdown-like phenotype with an extensive lag phase of growth. The null mutants are also unable to establish a sustained infection in a mouse model
Leishmania infantum
3.5.1.19
physiological function
Leishmania nicotinamidase is essential for NAD+ production and parasite proliferation
Leishmania infantum
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.1.19
malfunction
the generation of nicotinamidase null mutants leads to a decrease in NAD+ content, associated with a metabolic shutdown-like phenotype with an extensive lag phase of growth. The null mutants are also unable to establish a sustained infection in a mouse model
Leishmania infantum
3.5.1.19
physiological function
Leishmania nicotinamidase is essential for NAD+ production and parasite proliferation
Leishmania infantum