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Literature summary extracted from

  • Wang, X.D.; Gu, J.; Wang, T.; Bi, L.J.; Zhang, Z.P.; Cui, Z.Q.; Wei, H.P.; Deng, J.Y.; Zhang, X.E.
    Comparative analysis of mycobacterial NADH pyrophosphatase isoforms reveals a novel mechanism for isoniazid and ethionamide inactivation (2011), Mol. Microbiol., 82, 1375-1391.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.22 expressed in Escherichia coli BL21 (DE3) cells Mycobacterium tuberculosis
3.6.1.22 expressed in Escherichia coli BL21 (DE3) cells and in Mycobacterium smegmatis strain mc2155 Mycobacterium tuberculosis variant bovis

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.22 P237Q the mutation prevents dimer formation, and results in a loss of enzymatic activity Mycobacterium tuberculosis variant bovis
3.6.1.22 P237Q the mutation prevents dimer formation, and results in a loss of enzymatic activity Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.22 Ca2+ no activity in the presence of Ca2+ Mycobacterium tuberculosis
3.6.1.22 Ca2+ no activity in the presence of Ca2+ Mycobacterium tuberculosis variant bovis
3.6.1.22 Cu2+ no activity in the presence of Cu2+ Mycobacterium tuberculosis
3.6.1.22 Cu2+ no activity in the presence of Cu2+ Mycobacterium tuberculosis variant bovis
3.6.1.22 Fe2+ no activity in the presence of Fe2+ Mycobacterium tuberculosis
3.6.1.22 Fe3+ no activity in the presence of Fe3+ Mycobacterium tuberculosis
3.6.1.22 Fe3+ no activity in the presence of Fe3+ Mycobacterium tuberculosis variant bovis
3.6.1.22 Mg2+ no activity in the presence of Mg2+ Mycobacterium tuberculosis
3.6.1.22 Zn2+ no activity in the presence of Zn2+ Mycobacterium tuberculosis
3.6.1.22 Zn2+ no activity in the presence of Zn2+ Mycobacterium tuberculosis variant bovis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.22 Fe2+ the enzyme shows some activity on NADH in the presence of Fe2+ Mycobacterium tuberculosis variant bovis
3.6.1.22 Mg2+ Mg2+ has highest stimulating effect Mycobacterium tuberculosis variant bovis
3.6.1.22 Mn2+ Mn2+ has highest stimulating effect Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.22 40000
-
 2 * 40000, SDS-PAGE Mycobacterium tuberculosis variant bovis
3.6.1.22 50000
-
 gel filtration Mycobacterium tuberculosis
3.6.1.22 51000
-
 1 * 51000, purified recombinant NudC (fused with Trx tag), calculated from amino acid sequence Mycobacterium tuberculosis
3.6.1.22 80000
-
 gel filtration Mycobacterium tuberculosis variant bovis

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.22 Mycobacterium tuberculosis P9WIX5
-
-
3.6.1.22 Mycobacterium tuberculosis H37Rv P9WIX5
-
-
3.6.1.22 Mycobacterium tuberculosis variant bovis C1AGW8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.22 nickel affinity column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis variant bovis
3.6.1.22 nickel affinity column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.22 ethionamide-NAD+ + H2O
-
 Mycobacterium tuberculosis variant bovis ethionamide-NMNH + AMP
-
 ?
3.6.1.22 isoniazid-NAD+ + H2O
-
 Mycobacterium tuberculosis variant bovis isoniazid-NMNH + AMP
-
 ?
3.6.1.22 NAD+ + H2O weak substrate Mycobacterium tuberculosis variant bovis NMN + AMP
-
 ?
3.6.1.22 NAD+ + H2O weak substrate Mycobacterium tuberculosis NMN + AMP
-
 ?
3.6.1.22 NAD+ + H2O weak substrate Mycobacterium tuberculosis H37Rv NMN + AMP
-
 ?
3.6.1.22 NADH + H2O highest activity with NADH Mycobacterium tuberculosis variant bovis NMNH + AMP
-
 ?
3.6.1.22 NADH + H2O highest activity with NADH Mycobacterium tuberculosis NMNH + AMP
-
 ?
3.6.1.22 NADH + H2O highest activity with NADH Mycobacterium tuberculosis H37Rv NMNH + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.22 homodimer 2 * 40000, SDS-PAGE Mycobacterium tuberculosis variant bovis
3.6.1.22 monomer 1 * 50000, SDS-PAGE Mycobacterium tuberculosis
3.6.1.22 monomer 1 * 51000, purified recombinant NudC (fused with Trx tag), calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.6.1.22 NADH pyrophosphatase
-
 Mycobacterium tuberculosis variant bovis
3.6.1.22 NADH pyrophosphatase
-
 Mycobacterium tuberculosis
3.6.1.22 NudC
-
 Mycobacterium tuberculosis variant bovis
3.6.1.22 NudC
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.22 40
-
-
 Mycobacterium tuberculosis variant bovis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.1.22 35 45 enzyme activity rapidly decreases below 35°C or above 45°C Mycobacterium tuberculosis variant bovis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.22 8
-
 enzyme activity rapidly decreases below pH 7.5 Mycobacterium tuberculosis variant bovis

General Information

EC Number General Information Comment Organism
3.6.1.22 malfunction deletion of the NADH pyrophosphatase gene nudC results in increased susceptibility to isoniazid and ethionamide Mycobacterium tuberculosis variant bovis
3.6.1.22 physiological function NADH pyrophosphatase NudC plays an important role in the inactivation of isoniazid and ethionamide Mycobacterium tuberculosis variant bovis