Literature summary extracted from

Hebecker, S.; Arendt, W.; Heinemann, I.U.; Tiefenau, J.H.; Nimtz, M.; Rohde, M.; Soell, D.; Moser, J.
Alanyl-phosphatidylglycerol synthase: mechanism of substrate recognition during tRNA-dependent lipid modification in Pseudomonas aeruginosa (2011), Mol. Microbiol., 80, 935-950.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.3.2.11	enzymatically active residues 543-881 N-terminally fused to glutathione S-transferase are expressed in Escherichia coli BL21(lambdaDE3) cells	Pseudomonas aeruginosa

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.3.2.11	D579A	the mutant shows decreased activity (3%) compared to the wild type enzyme	Pseudomonas aeruginosa
2.3.2.11	D579N	the mutant shows decreased activity (5%) compared to the wild type enzyme	Pseudomonas aeruginosa
2.3.2.11	D710A	the mutant has 26% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	D710N	the mutant has 30% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	D765A	inactive	Pseudomonas aeruginosa
2.3.2.11	D765N	inactive	Pseudomonas aeruginosa
2.3.2.11	E657D	the mutant shows decreased activity (4%) compared to the wild type enzyme	Pseudomonas aeruginosa
2.3.2.11	E657Q	the mutant has 21% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	E658D	the mutant has 28% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	E658Q	the mutant has 19% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	E720Q	the mutant has 10% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	K580Q	inactive	Pseudomonas aeruginosa
2.3.2.11	K580S	inactive	Pseudomonas aeruginosa
2.3.2.11	K654Q	the mutant has 65% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	K654S	the mutant has 46% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	K840Q	inactive	Pseudomonas aeruginosa
2.3.2.11	K840S	inactive	Pseudomonas aeruginosa
2.3.2.11	K842Q	the mutant has wild type activity	Pseudomonas aeruginosa
2.3.2.11	K842S	the mutant has 50% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	M778A	the mutant has 50% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	R768Q	inactive	Pseudomonas aeruginosa
2.3.2.11	R768S	inactive	Pseudomonas aeruginosa
2.3.2.11	R837Q	the mutant has wild type activity	Pseudomonas aeruginosa
2.3.2.11	R837S	the mutant has 65% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	S709A	the mutant has 5% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	S709N	the mutant has 17% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	S724A	the mutant shows decreased activity (7%) compared to the wild type enzyme	Pseudomonas aeruginosa
2.3.2.11	S724N	inactive	Pseudomonas aeruginosa
2.3.2.11	S763A	the mutant has 23% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	S763N	the mutant has 11% of wild type activity	Pseudomonas aeruginosa
2.3.2.11	Y635A	inactive	Pseudomonas aeruginosa
2.3.2.11	Y732A	the mutant shows decreased activity (3%) compared to the wild type enzyme	Pseudomonas aeruginosa
2.3.2.11	Y831A	inactive	Pseudomonas aeruginosa

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.3.2.11	1,10-phenanthroline	20% residual activity at 20 mM	Pseudomonas aeruginosa
2.3.2.11	additional information	in the presence of up to 20 mM EDTA or up to 5 mM 1,10-phenanthroline no significant inactivation is observed	Pseudomonas aeruginosa

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.3.2.11	membrane	a transmembrane protein which is located in the inner membrane	Pseudomonas aeruginosa	16020	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.3.2.11	additional information	enzyme catalysis is not dependent on metal ions	Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.3.2.11	34000	-	gel filtration	Pseudomonas aeruginosa
2.3.2.11	38000	-	1 * 38000, SDS-PAGE	Pseudomonas aeruginosa
2.3.2.11	38720	-	1 * 38720, calculated from amino acid sequence	Pseudomonas aeruginosa

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.2.11	Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.3.2.11	glutathione Sepharose column chromatography	Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2.3.2.11	L-alanyl-tRNA + 1,2-dicaproyl-sn-glycero-3-phospho-(1'-rac-glycerol)	20% activity compared to phosphatidylglycerol	Pseudomonas aeruginosa	?	-	?
2.3.2.11	L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)	100% activity compared to phosphatidylglycerol	Pseudomonas aeruginosa	?	-	?
2.3.2.11	L-alanyl-tRNA + 1-oleoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol)	20% activity compared to phosphatidylglycerol	Pseudomonas aeruginosa	?	-	?
2.3.2.11	L-alanyl-tRNAAla + phosphatidylglycerol	100% activity	Pseudomonas aeruginosa	tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol	-	?
2.3.2.11	microhelix A + phosphatidylglycerol	90% activity compared to L-alanyl-tRNA	Pseudomonas aeruginosa	?	-	?
2.3.2.11	microhelix B + phosphatidylglycerol	40% activity compared to L-alanyl-tRNA	Pseudomonas aeruginosa	?	-	?
2.3.2.11	microhelix C + phosphatidylglycerol	-	Pseudomonas aeruginosa	?	-	?
2.3.2.11	microhelix D + phosphatidylglycerol	-	Pseudomonas aeruginosa	?	-	?
2.3.2.11	microhelix E + phosphatidylglycerol	90% activity compared to L-alanyl-tRNA	Pseudomonas aeruginosa	?	-	?
2.3.2.11	additional information	1,2-diplamitoyl-sn-glycero-3-phospho(ethylene glycol), microhelix F, N-acetylalanyl-alanyl-tRNA, lactyl-alanyl-tRNA and phenylalanyl-alanyl-tRNA are no substrates	Pseudomonas aeruginosa	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.3.2.11	monomer	1 * 38000, SDS-PAGE	Pseudomonas aeruginosa
2.3.2.11	monomer	1 * 38720, calculated from amino acid sequence	Pseudomonas aeruginosa

Synonyms

EC Number	Synonyms	Comment	Organism
2.3.2.11	A-PGS	-	Pseudomonas aeruginosa
2.3.2.11	alanyl-phosphatidylglycerol synthase	-	Pseudomonas aeruginosa