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Literature summary extracted from

  • Che, X.; Hu, J.; Wang, L.; Zhu, Z.; Xu, Q.; Lv, J.; Fu, Z.; Sun, Y.; Sun, J.; Lin, G.; Lu, R.; Yao, Z.
    Expression, purification, and activity assay of peptide deformylase from Escherichia coli and Staphylococcus aureus (2011), Mol. Cell. Biochem., 357, 47-54.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.88 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

General Stability

EC Number General Stability Organism
3.5.1.88 PDF has high activity in Tris buffer, but low activity in HEPES and NaH2PO4 buffers Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.88 actinonin actinonin does exert a significant inhibitory effect on enzyme activity in the presence of 0.25 mM substrate (complete inhibition at 10 nM) Staphylococcus aureus
3.5.1.88 additional information there is no significant inhibitory effect of actinonin on enzyme activity when the concentration of substrate is 4 mM Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.88 Fe2+ the imidazole ring of histidine can combine with a metal ion such as iron. The activity of the iron form of the enzyme is higher than the nickel form Staphylococcus aureus
3.5.1.88 Ni2+ the imidazole ring of histidine can combine with a metal ion such as nickel. The activity of the iron form of the enzyme is higher than the nickel form Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.88 20000
-
x * 20000, SDS-PAGE Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.88 Staphylococcus aureus
-
-
-
3.5.1.88 Staphylococcus aureus Mu50
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.88 Q-Sepharose column chromatography and Sephacryl S-300 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.88 N-formyl-Met-Val-Ser + H2O
-
Staphylococcus aureus formate + Met-Val-Ser
-
?
3.5.1.88 N-formyl-Met-Val-Ser + H2O
-
Staphylococcus aureus Mu50 formate + Met-Val-Ser
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.88 ? x * 20000, SDS-PAGE Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.88 PDF
-
Staphylococcus aureus