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Literature summary extracted from

  • Mohanpuria, P.; Kumar, V.; Ahuja, P.S.; Yadav, S.K.
    Agrobacterium-mediated silencing of caffeine synthesis through root transformation in Camellia sinensis L. (2011), Mol. Biotechnol., 48, 235-243.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.160 Agrobacterium tumefaciens-mediated silencing of caffeine synthesis through root transformation in Camellia sinensis suign the RNAi construct, pFGC1008-CS, method, overview Camellia sinensis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.160 additional information Agrobacterium tumefaciens-mediated silencing of caffeine synthesis through root transformation in Camellia sinensis suign the RNAi construct, pFGC1008-CS. The pFGC1008-CS contained 376 bp of caffeine synthase cDNA fragment in sense and antisense direction with an intron in between. Marked reduction in caffeine and theobromine contents in young shoots of tea seedlings occur after root transformation Camellia sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.160 additional information Camellia sinensis tea caffeine synthase is a bifunctional enzyme comprising two S-adenosyl-L-methionine-dependent N-methyltransferase activities leading to the formation of theobromine and caffeine ?
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine Camellia sinensis
-
 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 7-methylxanthine Camellia sinensis
-
 S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.160 Camellia sinensis B9VI89 cv. Kangra jat
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.160 leaf
-
 Camellia sinensis
-
2.1.1.160 root
-
 Camellia sinensis
-
2.1.1.160 seedling
-
 Camellia sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.160 additional information tea caffeine synthase is a bifunctional enzyme comprising two S-adenosyl-L-methionine-dependent N-methyltransferase activities leading to the formation of theobromine and caffeine Camellia sinensis ?
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 7-methylxanthine
-
 Camellia sinensis S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.160 S-adenosyl-L-methionine
-
 Camellia sinensis