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Literature summary extracted from

  • Yang, J.; Adhikari, M.; Liu, H.; Xu, H.; He, G.; Zhan, R.; Wei, J.; Chen, W.
    Characterization and functional analysis of the genes encoding 1-deoxy-D-xylulose-5-phosphate reductoisomerase and 1-deoxy-D-xylulose-5-phosphate synthase, the two enzymes in the MEP pathway, from Amomum villosum Lour (2012), Mol. Biol. Rep., 39, 8287-8296.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 DNA and amino acid sequence determination and analysis, semi-quantitative RT-PCR expression pattern analysis, functional recombinant expression under the control of CaMV 35S promoter in Nicotiana tabacum cv. Zhongyan 90 via Agrobacterium-mediated transformation Overexpression of AvDXR in tobacco increases DXRactivity, photosynthetic pigment content and volatile isoprenoid components Wurfbainia villosa
2.2.1.7 expression in Escherichia coli Wurfbainia villosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.267 chloroplast a putative chloroplast transit peptide with a conserved cleavage site of CS-M motif is located at the N-terminus of AvDXR Wurfbainia villosa 9507
-
2.2.1.7 chloroplast sequence contains a chloroplast transit peptide Wurfbainia villosa 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Mg2+ required Wurfbainia villosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.7 77150
-
 x * 77150, calculated Wurfbainia villosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Wurfbainia villosa
-
 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Wurfbainia villosa C6G1Y8 from medicinal garden of Guangzhou University of Chinese Medicine, China
-
2.2.1.7 Wurfbainia villosa C6G1Y5
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.2.1.7 proteolytic modification sequence contains a chloroplast transit peptide Wurfbainia villosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.267 fruit higher enzyme level Wurfbainia villosa
-
1.1.1.267 leaf
-
 Wurfbainia villosa
-
1.1.1.267 additional information semi-quantitative RT-PCR expression pattern analysis Wurfbainia villosa
-
1.1.1.267 pericarp
-
 Wurfbainia villosa
-
1.1.1.267 root
-
 Wurfbainia villosa
-
1.1.1.267 seedling
-
 Wurfbainia villosa
-
1.1.1.267 stem
-
 Wurfbainia villosa
-
2.2.1.7 leaf extensively expressed Wurfbainia villosa
-
2.2.1.7 additional information expression level is similar in all tissues examined Wurfbainia villosa
-
2.2.1.7 pericarp extensively expressed Wurfbainia villosa
-
2.2.1.7 root extensively expressed Wurfbainia villosa
-
2.2.1.7 seed extensively expressed Wurfbainia villosa
-
2.2.1.7 stem extensively expressed Wurfbainia villosa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Wurfbainia villosa 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.7 ? x * 77150, calculated Wurfbainia villosa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 1-deoxy-D-xylulose-5-phosphate reductoisomerase
-
 Wurfbainia villosa
1.1.1.267 DXR
-
 Wurfbainia villosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.267 7.5
-
 assay at Wurfbainia villosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADPH the NADPH-binding domains are critical to the catalytic activity of DXR. Two highly conserved NADPH-binding domains, GSTGSIGT and LAAGSNV, locate in the N-terminal region of AvDXR Wurfbainia villosa
2.2.1.7 thiamine diphosphate sequence contains a thiamin diphosphate-binding domain in the N-terminus, which begins and concludes with the highly conserved sequences -GDG- and - LNDN-, respectively Wurfbainia villosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.2.1.7 Wurfbainia villosa calculated
-
 7.5

General Information

EC Number General Information Comment Organism
1.1.1.267 metabolism the enzyme catalyzes the second regulation step in the 2-C-methyl-D-erythritol 4-phosphate pathway and the the first committed step of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis Wurfbainia villosa
1.1.1.267 additional information two substrate-binding domains, LPADSEHSAI and NKGLEVIEAHY, locate in its mid-region of the enzyme molecule, which is highly homologous to DXRs from other plants and similar to the DXR from Escherichia coli Wurfbainia villosa