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Literature summary extracted from

  • Dimou, M.; Venieraki, A.; Liakopoulos, G.; Katinakis, P.
    Cloning, characterization and transcriptional analysis of two phosphate acetyltransferase isoforms from Azotobacter vinelandii (2011), Mol. Biol. Rep., 38, 3653-3663.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.8 expression in Escherichia coli Azotobacter vinelandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.8 acetyl phosphate substrate inhibition; substrate inhibition Azotobacter vinelandii
2.3.1.8 CoA substrate inhibition; substrate inhibition Azotobacter vinelandii
2.3.1.8 phosphate substrate inhibition; substrate inhibition Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.8 0.05
-
 acetyl-CoA pH 7.2, 35°C Azotobacter vinelandii
2.3.1.8 0.2
-
 CoA pH 7.2, 35°C Azotobacter vinelandii
2.3.1.8 0.3
-
 CoA pH 7.2, 35°C Azotobacter vinelandii
2.3.1.8 0.5
-
 acetyl phosphate pH 7.2, 35°C Azotobacter vinelandii
2.3.1.8 1
-
 acetyl phosphate pH 7.2, 35°C Azotobacter vinelandii
2.3.1.8 11
-
 phosphate pH 7.2, 35°C Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.8 76000
-
 x * 76000, SDS-PAGE, recombinant protein Azotobacter vinelandii
2.3.1.8 77000
-
 x * 77000, SDS-PAGE, recombinant protein Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Azotobacter vinelandii C1DER7 isoform Pta-2
-
2.3.1.8 Azotobacter vinelandii C1DQG8 isoform Pta-1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl phosphate + CoA
-
 Azotobacter vinelandii acetyl-CoA + phosphate
-
 ?
2.3.1.8 acetyl phosphate + CoA with isoform Pta-2, reaction is irreversible, no acetyl phosphate forming reaction can be detected Azotobacter vinelandii acetyl-CoA + phosphate
-
 r
2.3.1.8 acetyl-CoA + phosphate
-
 Azotobacter vinelandii acetyl Phosphate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.8 ? x * 76000, SDS-PAGE, recombinant protein Azotobacter vinelandii
2.3.1.8 ? x * 77000, SDS-PAGE, recombinant protein Azotobacter vinelandii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.8 0.04
-
 CoA pH 7.2, 35°C Azotobacter vinelandii
2.3.1.8 0.1
-
 CoA pH 7.2, 35°C Azotobacter vinelandii
2.3.1.8 5.2
-
 acetyl phosphate pH 7.2, 35°C Azotobacter vinelandii
2.3.1.8 12
-
 acetyl phosphate pH 7.2, 35°C Azotobacter vinelandii
2.3.1.8 19
-
 phosphate pH 7.2, 35°C Azotobacter vinelandii

Expression

EC Number Organism Comment Expression
2.3.1.8 Azotobacter vinelandii gene is expressed during exponential growth on glucose or acetate and is downregulated in the stationary phase additional information
2.3.1.8 Azotobacter vinelandii availabity of ammonium during growth on acetate results in upregulation up