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Literature summary extracted from

  • Chanama, M.; Chanama, S.; Shaw, P.J.; Chitnumsub, P.; Leartsakulpanich, U.; Yuthavong, Y.
    Formation of catalytically active cross-species heterodimers of thymidylate synthase from Plasmodium falciparum and Plasmodium vivax (2011), Mol. Biol. Rep., 38, 1029-1037.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 expressed in Escherichia coli chi2913RecA and BL21(DE3)pLysS cells Plasmodium falciparum
2.1.1.45 expressed in Escherichia coli chi2913RecA and BL21(DE3)pLysS cells Plasmodium vivax

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.45 C490A inactive Plasmodium falciparum
2.1.1.45 C506A inactive Plasmodium vivax
2.1.1.45 C506D inactive Plasmodium vivax
2.1.1.45 C506F inactive Plasmodium vivax
2.1.1.45 C506P inactive Plasmodium vivax
2.1.1.45 C506Y inactive Plasmodium vivax
2.1.1.45 R470D inactive Plasmodium falciparum
2.1.1.45 R486D inactive Plasmodium vivax
2.1.1.45 R486L inactive Plasmodium vivax
2.1.1.45 R486Y inactive Plasmodium vivax

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Plasmodium falciparum
-
-
-
2.1.1.45 Plasmodium vivax
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 methotrexate-Sepharose column chromatography and anion exchange column chromatography Plasmodium falciparum
2.1.1.45 methotrexate-Sepharose column chromatography and anion exchange column chromatography Plasmodium vivax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Plasmodium falciparum dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Plasmodium vivax dihydrofolate + dTMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 homodimer
-
 Plasmodium falciparum
2.1.1.45 homodimer
-
 Plasmodium vivax