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Literature summary extracted from

  • Figueiredo, J.M.; Rodrigues, D.C.; Silva, R.C.; Koeller, C.M.; Jiang, J.C.; Jazwinski, S.M.; Previato, J.O.; Mendonca-Previato, L.; Urmenyi, T.P.; Heise, N.
    Molecular and functional characterization of the ceramide synthase from Trypanosoma cruzi (2012), Mol. Biochem. Parasitol., 182, 62-74.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.24 CHAPS required Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.24 fumonisin B1
-
Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.24 0.0124
-
palmitoyl-CoA pH 7.5, 28°C Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.24 microsome no reverse ceramidase activity is detected in Trypanosoma cruzi microsomal fractions Trypanosoma cruzi
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.24 additional information the change of a Tris-Mg2+-DTT-NaF-NADPH-CHAPS buffer to a phosphate/K+-Mn2+-CHAPS buffer slightly improves the efficiency of Cer formation Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Trypanosoma cruzi G0YYC4 Trypanosoma cruzi microsomes harbor a Fumonisin B1-sensitive acyl-CoA-dependent CerS activity that uses both dihydrosphingosine and sphingosine as long-chain sphingoid base acceptors and palmitoyl-CoA as the principal fatty-acyl-CoA substrate donor
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 additional information palmitoyl-CoAis the preferred substrate. Low activity towards myristoyl-CoA and stearoyl-CoA, and almost no CerS activity associated with all other acyl-CoAs tested, which range from C3, C4 and to C20 up to C26 Trypanosoma cruzi ?
-
?
2.3.1.24 palmitoyl-CoA + dihydrosphingosine no reverse ceramidase activity is detected in Trypanosoma cruzi microsomal fractions Trypanosoma cruzi CoA + N-palmitoyldihydrosphingosine
-
?
2.3.1.24 palmitoyl-CoA + sphingosine no reverse ceramidase activity is detected in Trypanosoma cruzi microsomal fractions Trypanosoma cruzi CoA + N-palmitoylsphingosine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.24 CerS
-
Trypanosoma cruzi
2.3.1.24 TcCerS
-
Trypanosoma cruzi
2.3.1.24 TcCERS1
-
Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.24 14 28 TcCerS from Y strain has an optimal activity that increases between 14°C and 28°C, but decreases at 37°C and 42°C Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.24 7.5
-
assay at Trypanosoma cruzi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.24 7 7.5 optimal pH Trypanosoma cruzi

General Information

EC Number General Information Comment Organism
2.3.1.24 malfunction the TcCERS1 gene is able to functionally complement the lethality of a lag1DELTA lac1DELTA double deletion yeast mutant in which the acyl-CoA-dependent CerS is not detectable Trypanosoma cruzi