EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.24 | CHAPS | required | Trypanosoma cruzi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.24 | fumonisin B1 | - |
Trypanosoma cruzi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.24 | 0.0124 | - |
palmitoyl-CoA | pH 7.5, 28°C | Trypanosoma cruzi |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.24 | microsome | no reverse ceramidase activity is detected in Trypanosoma cruzi microsomal fractions | Trypanosoma cruzi | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.24 | additional information | the change of a Tris-Mg2+-DTT-NaF-NADPH-CHAPS buffer to a phosphate/K+-Mn2+-CHAPS buffer slightly improves the efficiency of Cer formation | Trypanosoma cruzi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.24 | Trypanosoma cruzi | G0YYC4 | Trypanosoma cruzi microsomes harbor a Fumonisin B1-sensitive acyl-CoA-dependent CerS activity that uses both dihydrosphingosine and sphingosine as long-chain sphingoid base acceptors and palmitoyl-CoA as the principal fatty-acyl-CoA substrate donor | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.24 | additional information | palmitoyl-CoAis the preferred substrate. Low activity towards myristoyl-CoA and stearoyl-CoA, and almost no CerS activity associated with all other acyl-CoAs tested, which range from C3, C4 and to C20 up to C26 | Trypanosoma cruzi | ? | - |
? | |
2.3.1.24 | palmitoyl-CoA + dihydrosphingosine | no reverse ceramidase activity is detected in Trypanosoma cruzi microsomal fractions | Trypanosoma cruzi | CoA + N-palmitoyldihydrosphingosine | - |
? | |
2.3.1.24 | palmitoyl-CoA + sphingosine | no reverse ceramidase activity is detected in Trypanosoma cruzi microsomal fractions | Trypanosoma cruzi | CoA + N-palmitoylsphingosine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.24 | CerS | - |
Trypanosoma cruzi |
2.3.1.24 | TcCerS | - |
Trypanosoma cruzi |
2.3.1.24 | TcCERS1 | - |
Trypanosoma cruzi |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.24 | 14 | 28 | TcCerS from Y strain has an optimal activity that increases between 14°C and 28°C, but decreases at 37°C and 42°C | Trypanosoma cruzi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.24 | 7.5 | - |
assay at | Trypanosoma cruzi |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.24 | 7 | 7.5 | optimal pH | Trypanosoma cruzi |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.24 | malfunction | the TcCERS1 gene is able to functionally complement the lethality of a lag1DELTA lac1DELTA double deletion yeast mutant in which the acyl-CoA-dependent CerS is not detectable | Trypanosoma cruzi |