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Literature summary extracted from

  • Stoffel, S.A.; Alibu, V.P.; Hubert, J.; Ebikeme, C.; Portais, J.C.; Bringaud, F.; Schweingruber, M.E.; Barrett, M.P.
    Transketolase in Trypanosoma brucei (2011), Mol. Biochem. Parasitol., 179, 1-7.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.1 expression in Escherichia coli Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 0.2
-
D-xylulose 5-phosphate pH 7.5, 22°C Trypanosoma brucei
2.2.1.1 0.8
-
D-ribose 5-phosphate pH 7.5, 22°C Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.2.1.1 cytosol main localization Trypanosoma brucei 5829
-
2.2.1.1 glycosome to lesser extent than in cytosol Trypanosoma brucei 20015
-

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1 recombinant enzyme Trypanosoma brucei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.2.1.1 additional information transketolase activity is absent from the long slender bloodstream form of the parasite and the protein is not detectable in this life cycle stage, with the RNA present only at low abundance Trypanosoma brucei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
Trypanosoma brucei sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
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General Information

EC Number General Information Comment Organism
2.2.1.1 physiological function gene knock out from procyclic Trypanosoma brucei results in loss of transketolase activity ,but no growth phenotype is evident in the null mutants. Metabolite profiling to compare wild type and TKT null mutants reveals substantial increases in transketolase substrate metabolites coupled to loss of sedoheptulose 7-phosphate Trypanosoma brucei