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Literature summary extracted from

  • Wallwey, C.; Heddergott, C.; Xie, X.; Brakhage, A.A.; Li, S.M.
    Genome mining reveals the presence of a conserved gene cluster for the biosynthesis of ergot alkaloid precursors in the fungal family Arthrodermataceae (2012), Microbiology, 158, 1634-1644.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.332 expression in Escherichia coli Trichophyton benhamiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.332 EDTA 5 mM, 71% of initial activity Trichophyton benhamiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.332 0.09
-
 chanoclavine-I pH 7.5, 37°C Trichophyton benhamiae
1.1.1.332 0.36
-
 NAD+ pH 7.5, 37°C Trichophyton benhamiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.332 Co2+ 5 mM, addition to EDTA-treated enzyme, 31% of control activity Trichophyton benhamiae
1.1.1.332 Mn2+ 5 mM, addition to EDTA-treated enzyme, 35% of control activity Trichophyton benhamiae
1.1.1.332 Ni2+ 5 mM, addition to EDTA-treated enzyme, 73% of control activity Trichophyton benhamiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.332 26200
-
 4 * 26200, calculated, 4 * 30000, SDS-PAGE of recombinant His-tagged protein Trichophyton benhamiae
1.1.1.332 30000
-
 4 * 26200, calculated, 4 * 30000, SDS-PAGE of recombinant His-tagged protein Trichophyton benhamiae
1.1.1.332 128000
-
 gel filtration Trichophyton benhamiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.332 Trichophyton benhamiae D4AK45
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.332 chanoclavine-I + NAD+
-
 Trichophyton benhamiae chanoclavine-I aldehyde + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.332 tetramer 4 * 26200, calculated, 4 * 30000, SDS-PAGE of recombinant His-tagged protein Trichophyton benhamiae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.332 ARB_04646
-
 Trichophyton benhamiae
1.1.1.332 ChaDH
-
 Trichophyton benhamiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.332 0.76
-
 chanoclavine-I pH 7.5, 37°C Trichophyton benhamiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.332 additional information no cofactor: NADP+, FMN or FAD Trichophyton benhamiae
1.1.1.332 NAD+ specific for NAD+ Trichophyton benhamiae