EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.3.3 | gene lmo0036, phylogenetic tree, quantitative real-time PCR expresion analysis at different acidic conditions, overview | Listeria monocytogenes |
2.1.3.6 | gene lmo0036, phylogenetic tree, quantitative real-time PCR expresion analysis at different acidic conditions, overview | Listeria monocytogenes |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.3.3 | additional information | construction of a lmo0036 knockout mutant by homologous recombination using for in-frame deletion of the whole length of lmo0036 | Listeria monocytogenes |
2.1.3.6 | additional information | construction of a lmo0036 knockout mutant by homologous recombination using for in-frame deletion of the whole length of lmo0036 | Listeria monocytogenes |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.3 | carbamoyl phosphate + L-ornithine | Listeria monocytogenes | - |
L-citrulline + phosphate | - |
r | |
2.1.3.3 | carbamoyl phosphate + L-ornithine | Listeria monocytogenes 10403S | - |
L-citrulline + phosphate | - |
r | |
2.1.3.3 | additional information | Listeria monocytogenes | Lmo0036 is an ornithine carbamoyltransferase and also a putrescine carbamoyltransferase, EC 2.1.3.6, catalysing reversible ornithine and putrescine carbamoyltransfer reactions | ? | - |
? | |
2.1.3.3 | additional information | Listeria monocytogenes 10403S | Lmo0036 is an ornithine carbamoyltransferase and also a putrescine carbamoyltransferase, EC 2.1.3.6, catalysing reversible ornithine and putrescine carbamoyltransfer reactions | ? | - |
? | |
2.1.3.6 | carbamoyl phosphate + putrescine | Listeria monocytogenes | - |
phosphate + N-carbamoylputrescine | - |
r | |
2.1.3.6 | carbamoyl phosphate + putrescine | Listeria monocytogenes 10403S | - |
phosphate + N-carbamoylputrescine | - |
r | |
2.1.3.6 | additional information | Listeria monocytogenes | Lmo0036 is an ornithine carbamoyltransferase, EC 2.1.3.3, and also a putrescine carbamoyltransferase, catalysing reversible ornithine and putrescine carbamoyltransfer reactions | ? | - |
? | |
2.1.3.6 | additional information | Listeria monocytogenes 10403S | Lmo0036 is an ornithine carbamoyltransferase, EC 2.1.3.3, and also a putrescine carbamoyltransferase, catalysing reversible ornithine and putrescine carbamoyltransfer reactions | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.3.3 | Listeria monocytogenes | - |
gene lmo0036 | - |
2.1.3.3 | Listeria monocytogenes 10403S | - |
gene lmo0036 | - |
2.1.3.6 | Listeria monocytogenes | - |
gene lmo0036 | - |
2.1.3.6 | Listeria monocytogenes 10403S | - |
gene lmo0036 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 0.12 | - |
catabolic reaction, pH 5.0-6.0, 37°C | Listeria monocytogenes |
2.1.3.3 | 3.59 | - |
anabolic reaction, pH 10.0, 37°C | Listeria monocytogenes |
2.1.3.6 | 0.57 | - |
anabolic reaction, pH 8.0, 37°C | Listeria monocytogenes |
2.1.3.6 | 35.6 | - |
catabolic reaction, pH 5.0, 37°C | Listeria monocytogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.3 | carbamoyl phosphate + L-ornithine | - |
Listeria monocytogenes | L-citrulline + phosphate | - |
r | |
2.1.3.3 | carbamoyl phosphate + L-ornithine | - |
Listeria monocytogenes 10403S | L-citrulline + phosphate | - |
r | |
2.1.3.3 | additional information | Lmo0036 is an ornithine carbamoyltransferase and also a putrescine carbamoyltransferase, EC 2.1.3.6, catalysing reversible ornithine and putrescine carbamoyltransfer reactions | Listeria monocytogenes | ? | - |
? | |
2.1.3.3 | additional information | Lmo0036 is an ornithine carbamoyltransferase and also a putrescine carbamoyltransferase, EC 2.1.3.6, catalysing reversible ornithine and putrescine carbamoyltransfer reactions | Listeria monocytogenes 10403S | ? | - |
? | |
2.1.3.6 | carbamoyl phosphate + putrescine | - |
Listeria monocytogenes | phosphate + N-carbamoylputrescine | - |
r | |
2.1.3.6 | carbamoyl phosphate + putrescine | - |
Listeria monocytogenes 10403S | phosphate + N-carbamoylputrescine | - |
r | |
2.1.3.6 | additional information | Lmo0036 is an ornithine carbamoyltransferase, EC 2.1.3.3, and also a putrescine carbamoyltransferase, catalysing reversible ornithine and putrescine carbamoyltransfer reactions | Listeria monocytogenes | ? | - |
? | |
2.1.3.6 | additional information | Lmo0036 is an ornithine carbamoyltransferase, EC 2.1.3.3, and also a putrescine carbamoyltransferase, catalysing reversible ornithine and putrescine carbamoyltransfer reactions | Listeria monocytogenes 10403S | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.3.3 | anabolic ornithine carbamoyltransferase | - |
Listeria monocytogenes |
2.1.3.3 | AOTC | - |
Listeria monocytogenes |
2.1.3.3 | catabolic ornithine carbamoyltransferase | - |
Listeria monocytogenes |
2.1.3.3 | cOTC | - |
Listeria monocytogenes |
2.1.3.3 | Lmo0036 | - |
Listeria monocytogenes |
2.1.3.3 | ornithine carbamoyltransferase | - |
Listeria monocytogenes |
2.1.3.6 | anabolic putrescine carbamoyltransferase | - |
Listeria monocytogenes |
2.1.3.6 | aPTC | - |
Listeria monocytogenes |
2.1.3.6 | catabolic putrescine carbamoyltransferase | - |
Listeria monocytogenes |
2.1.3.6 | cPTC | - |
Listeria monocytogenes |
2.1.3.6 | Lmo0036 | - |
Listeria monocytogenes |
2.1.3.6 | putrescine carbamoyltransferase | - |
Listeria monocytogenes |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 37 | - |
assay at | Listeria monocytogenes |
2.1.3.6 | 37 | - |
assay at | Listeria monocytogenes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 5 | 6 | catabolic reaction | Listeria monocytogenes |
2.1.3.3 | 10 | - |
anabolic reaction | Listeria monocytogenes |
2.1.3.6 | 5 | - |
catabolic reaction | Listeria monocytogenes |
2.1.3.6 | 8 | - |
anabolic reaction | Listeria monocytogenes |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 4 | 11 | activity range, anabolic and catabolic reactions | Listeria monocytogenes |
2.1.3.6 | 3 | 6.5 | activity range, catabolic reaction | Listeria monocytogenes |
2.1.3.6 | 4 | 11 | activity range, anabolic reaction | Listeria monocytogenes |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.3.3 | malfunction | absence of this enzyme impairs the growth of Listeria under mild acidic conditions at pH 4.8 and reduced its survival in synthetic human gastric fluid at pH 2.5, corresponding to a loss in ammonia production | Listeria monocytogenes |
2.1.3.3 | metabolism | catabolic ornithine and putrescine carbamoyltransfer reactions constitute the second step of arginine deiminase and agmatine deiminase pathways. However, the equilibrium of in vitro carbamoyltransfer reactions is overwhelmingly towards the anabolic direction, suggesting that catabolic carbamoyltransferase is probably the limiting step of the pathways. lmo0036 is induced at the transcriptional level when Listeria monocytogenes is subjected to low-pH stress. Its expression product in Escherichia coli exhibits higher catabolic carbamoyltransfer activities under acidic conditions | Listeria monocytogenes |
2.1.3.3 | physiological function | Lmo0036 is responsible for acid tolerance at both sublethal and lethal pH levels and plays a possible role in Listeria virulence | Listeria monocytogenes |
2.1.3.6 | malfunction | absence of this enzyme impairs the growth of Listeria under mild acidic conditions at pH 4.8 and reduced its survival in synthetic human gastric fluid at pH 2.5, corresponding to a loss in ammonia production | Listeria monocytogenes |
2.1.3.6 | metabolism | catabolic ornithine and putrescine carbamoyltransfer reactions constitute the second step of arginine deiminase and agmatine deiminase pathways. However, the equilibrium of in vitro carbamoyltransfer reactions is overwhelmingly towards the anabolic direction, suggesting that catabolic carbamoyltransferase is probably the limiting step of the pathways. lmo0036 is induced at the transcriptional level when Listeria monocytogenes is subjected to low-pH stress. Its expression product in Escherichia coli exhibits higher catabolic carbamoyltransfer activities under acidic conditions | Listeria monocytogenes |
2.1.3.6 | physiological function | Lmo0036 is responsible for acid tolerance at both sublethal and lethal pH levels and plays a possible role in Listeria virulence | Listeria monocytogenes |