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Literature summary extracted from

  • Chen, J.; Cheng, C.; Xia, Y.; Zhao, H.; Fang, C.; Shan, Y.; Wu, B.; Fang, W.
    Lmo0036, an ornithine and putrescine carbamoyltransferase in Listeria monocytogenes, participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance (2011), Microbiology, 157, 3150-3161.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.3 gene lmo0036, phylogenetic tree, quantitative real-time PCR expresion analysis at different acidic conditions, overview Listeria monocytogenes
2.1.3.6 gene lmo0036, phylogenetic tree, quantitative real-time PCR expresion analysis at different acidic conditions, overview Listeria monocytogenes

Protein Variants

EC Number Protein Variants Comment Organism
2.1.3.3 additional information construction of a lmo0036 knockout mutant by homologous recombination using for in-frame deletion of the whole length of lmo0036 Listeria monocytogenes
2.1.3.6 additional information construction of a lmo0036 knockout mutant by homologous recombination using for in-frame deletion of the whole length of lmo0036 Listeria monocytogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine Listeria monocytogenes
-
L-citrulline + phosphate
-
r
2.1.3.3 carbamoyl phosphate + L-ornithine Listeria monocytogenes 10403S
-
L-citrulline + phosphate
-
r
2.1.3.3 additional information Listeria monocytogenes Lmo0036 is an ornithine carbamoyltransferase and also a putrescine carbamoyltransferase, EC 2.1.3.6, catalysing reversible ornithine and putrescine carbamoyltransfer reactions ?
-
?
2.1.3.3 additional information Listeria monocytogenes 10403S Lmo0036 is an ornithine carbamoyltransferase and also a putrescine carbamoyltransferase, EC 2.1.3.6, catalysing reversible ornithine and putrescine carbamoyltransfer reactions ?
-
?
2.1.3.6 carbamoyl phosphate + putrescine Listeria monocytogenes
-
phosphate + N-carbamoylputrescine
-
r
2.1.3.6 carbamoyl phosphate + putrescine Listeria monocytogenes 10403S
-
phosphate + N-carbamoylputrescine
-
r
2.1.3.6 additional information Listeria monocytogenes Lmo0036 is an ornithine carbamoyltransferase, EC 2.1.3.3, and also a putrescine carbamoyltransferase, catalysing reversible ornithine and putrescine carbamoyltransfer reactions ?
-
?
2.1.3.6 additional information Listeria monocytogenes 10403S Lmo0036 is an ornithine carbamoyltransferase, EC 2.1.3.3, and also a putrescine carbamoyltransferase, catalysing reversible ornithine and putrescine carbamoyltransfer reactions ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Listeria monocytogenes
-
gene lmo0036
-
2.1.3.3 Listeria monocytogenes 10403S
-
gene lmo0036
-
2.1.3.6 Listeria monocytogenes
-
gene lmo0036
-
2.1.3.6 Listeria monocytogenes 10403S
-
gene lmo0036
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 0.12
-
catabolic reaction, pH 5.0-6.0, 37°C Listeria monocytogenes
2.1.3.3 3.59
-
anabolic reaction, pH 10.0, 37°C Listeria monocytogenes
2.1.3.6 0.57
-
anabolic reaction, pH 8.0, 37°C Listeria monocytogenes
2.1.3.6 35.6
-
catabolic reaction, pH 5.0, 37°C Listeria monocytogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
Listeria monocytogenes L-citrulline + phosphate
-
r
2.1.3.3 carbamoyl phosphate + L-ornithine
-
Listeria monocytogenes 10403S L-citrulline + phosphate
-
r
2.1.3.3 additional information Lmo0036 is an ornithine carbamoyltransferase and also a putrescine carbamoyltransferase, EC 2.1.3.6, catalysing reversible ornithine and putrescine carbamoyltransfer reactions Listeria monocytogenes ?
-
?
2.1.3.3 additional information Lmo0036 is an ornithine carbamoyltransferase and also a putrescine carbamoyltransferase, EC 2.1.3.6, catalysing reversible ornithine and putrescine carbamoyltransfer reactions Listeria monocytogenes 10403S ?
-
?
2.1.3.6 carbamoyl phosphate + putrescine
-
Listeria monocytogenes phosphate + N-carbamoylputrescine
-
r
2.1.3.6 carbamoyl phosphate + putrescine
-
Listeria monocytogenes 10403S phosphate + N-carbamoylputrescine
-
r
2.1.3.6 additional information Lmo0036 is an ornithine carbamoyltransferase, EC 2.1.3.3, and also a putrescine carbamoyltransferase, catalysing reversible ornithine and putrescine carbamoyltransfer reactions Listeria monocytogenes ?
-
?
2.1.3.6 additional information Lmo0036 is an ornithine carbamoyltransferase, EC 2.1.3.3, and also a putrescine carbamoyltransferase, catalysing reversible ornithine and putrescine carbamoyltransfer reactions Listeria monocytogenes 10403S ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.3.3 anabolic ornithine carbamoyltransferase
-
Listeria monocytogenes
2.1.3.3 AOTC
-
Listeria monocytogenes
2.1.3.3 catabolic ornithine carbamoyltransferase
-
Listeria monocytogenes
2.1.3.3 cOTC
-
Listeria monocytogenes
2.1.3.3 Lmo0036
-
Listeria monocytogenes
2.1.3.3 ornithine carbamoyltransferase
-
Listeria monocytogenes
2.1.3.6 anabolic putrescine carbamoyltransferase
-
Listeria monocytogenes
2.1.3.6 aPTC
-
Listeria monocytogenes
2.1.3.6 catabolic putrescine carbamoyltransferase
-
Listeria monocytogenes
2.1.3.6 cPTC
-
Listeria monocytogenes
2.1.3.6 Lmo0036
-
Listeria monocytogenes
2.1.3.6 putrescine carbamoyltransferase
-
Listeria monocytogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.3.3 37
-
assay at Listeria monocytogenes
2.1.3.6 37
-
assay at Listeria monocytogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 5 6 catabolic reaction Listeria monocytogenes
2.1.3.3 10
-
anabolic reaction Listeria monocytogenes
2.1.3.6 5
-
catabolic reaction Listeria monocytogenes
2.1.3.6 8
-
anabolic reaction Listeria monocytogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.3.3 4 11 activity range, anabolic and catabolic reactions Listeria monocytogenes
2.1.3.6 3 6.5 activity range, catabolic reaction Listeria monocytogenes
2.1.3.6 4 11 activity range, anabolic reaction Listeria monocytogenes

General Information

EC Number General Information Comment Organism
2.1.3.3 malfunction absence of this enzyme impairs the growth of Listeria under mild acidic conditions at pH 4.8 and reduced its survival in synthetic human gastric fluid at pH 2.5, corresponding to a loss in ammonia production Listeria monocytogenes
2.1.3.3 metabolism catabolic ornithine and putrescine carbamoyltransfer reactions constitute the second step of arginine deiminase and agmatine deiminase pathways. However, the equilibrium of in vitro carbamoyltransfer reactions is overwhelmingly towards the anabolic direction, suggesting that catabolic carbamoyltransferase is probably the limiting step of the pathways. lmo0036 is induced at the transcriptional level when Listeria monocytogenes is subjected to low-pH stress. Its expression product in Escherichia coli exhibits higher catabolic carbamoyltransfer activities under acidic conditions Listeria monocytogenes
2.1.3.3 physiological function Lmo0036 is responsible for acid tolerance at both sublethal and lethal pH levels and plays a possible role in Listeria virulence Listeria monocytogenes
2.1.3.6 malfunction absence of this enzyme impairs the growth of Listeria under mild acidic conditions at pH 4.8 and reduced its survival in synthetic human gastric fluid at pH 2.5, corresponding to a loss in ammonia production Listeria monocytogenes
2.1.3.6 metabolism catabolic ornithine and putrescine carbamoyltransfer reactions constitute the second step of arginine deiminase and agmatine deiminase pathways. However, the equilibrium of in vitro carbamoyltransfer reactions is overwhelmingly towards the anabolic direction, suggesting that catabolic carbamoyltransferase is probably the limiting step of the pathways. lmo0036 is induced at the transcriptional level when Listeria monocytogenes is subjected to low-pH stress. Its expression product in Escherichia coli exhibits higher catabolic carbamoyltransfer activities under acidic conditions Listeria monocytogenes
2.1.3.6 physiological function Lmo0036 is responsible for acid tolerance at both sublethal and lethal pH levels and plays a possible role in Listeria virulence Listeria monocytogenes