Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Grzegorzewicz, A.E.; Ma, Y.; Jones, V.; Crick, D.; Liav, A.; McNeil, M.R.
    Development of a microtitre plate-based assay for lipid-linked glycosyltransferase products using the mycobacterial cell wall rhamnosyltransferase WbbL (2008), Microbiology, 154, 3724-3730.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.289 2-mercaptoethanol stimulates the enzyme activity Mycobacterium tuberculosis

Application

EC Number Application Comment Organism
2.4.1.289 drug development WbbL is a good target for the development of antituberculosis therapeutics Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.289 gene wbb, overexpression in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.289 cell wall
-
Mycobacterium tuberculosis 5618
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.289 additional information WbbL shows an absolute requirement for divalent cations Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.289 dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol Mycobacterium tuberculosis natural acceptor substrate GlcNAc-P-P-decaprenyl dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
?
2.4.1.289 dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol Mycobacterium tuberculosis H37Rv natural acceptor substrate GlcNAc-P-P-decaprenyl dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.289 Mycobacterium tuberculosis
-
gene wbbL
-
2.4.1.289 Mycobacterium tuberculosis H37Rv
-
gene wbbL
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.289 dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol kinetic analysis shows that WbbL uses a ternary complex mechanism reaction Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.289 dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
Mycobacterium tuberculosis dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
?
2.4.1.289 dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol natural acceptor substrate GlcNAc-P-P-decaprenyl Mycobacterium tuberculosis dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
?
2.4.1.289 dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
Mycobacterium tuberculosis H37Rv dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
?
2.4.1.289 dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol natural acceptor substrate GlcNAc-P-P-decaprenyl Mycobacterium tuberculosis H37Rv dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.289 cell wall rhamnosyltransferase
-
Mycobacterium tuberculosis
2.4.1.289 WbbL
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.289 30
-
assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.289 8.6
-
assay at Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.4.1.289 physiological function WbbL catalyzes the transfer of an alpha-L-rhamnopyranose residue from dTDP-Rha to decaprenyldiphosphoryl-alpha-D-N-acetyl glucosamine, GlcNAc-P-PDP, to form alpha-L-Rhap-(1->3)-alpha-D-GlcNAc-P-P-DP, which is then further elongated with Galf and Araf units and finally mycolylated and attached to the peptidoglycan. The enzyme is essential for Mycobacterium tuberculosis viability and is absent in eukaryotic cells Mycobacterium tuberculosis