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Literature summary extracted from

  • Wu, P.; Cisneros, G.A.; Hu, H.; Chaudret, R.; Hu, X.; Yang, W.
    Catalytic mechanism of 4-oxalocrotonate tautomerase: significances of protein-protein interactions on proton transfer pathways (2012), J. Phys. Chem. B, 116, 6889-6897.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.2.6 2-oxo-4-hexenedioate Pseudomonas putida ketonization process via dienol intermediate 2-hydroxymuconate and with Pro1 as a general base, one-proton transfer mechanism 2-oxo-3-hexenedioate
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Organism

EC Number Organism UniProt Comment Textmining
5.3.2.6 Pseudomonas putida
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Reaction

EC Number Reaction Comment Organism Reaction ID
5.3.2.6 (2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate catalytic reaction mechanism, detailed overview. Quantum mechanics/molecular mechanics simulation of proton transfer reaction and structure-function analysis, the ketonization mechanism in 4-OT depends on the enzymatic model used, detailed overview Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.2.6 2-oxo-4-hexenedioate ketonization process via dienol intermediate 2-hydroxymuconate and with Pro1 as a general base, one-proton transfer mechanism Pseudomonas putida 2-oxo-3-hexenedioate
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 ?

Subunits

EC Number Subunits Comment Organism
5.3.2.6 hexamer 4-OT has an amino-terminal proline and a beta-alpha-beta fold, three structural models of 4-OT, overview Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
5.3.2.6 4-OT
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 Pseudomonas putida
5.3.2.6 4-oxalocrotonate tautomerase
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 Pseudomonas putida

General Information

EC Number General Information Comment Organism
5.3.2.6 evolution 4-oxalocrotonate tautomerase is a member of the tautomerase superfamily Pseudomonas putida
5.3.2.6 metabolism 4-oxalocrotonate tautomerase is an essential enzyme in the degradative metabolism pathway occurring in the Krebs cycle Pseudomonas putida
5.3.2.6 physiological function 4-OT catalyzes the ketonization process of 2-oxo-4-hexenedioate to its conjugated isomer, 2-oxo-3-hexadienedioate, through the dienol intermediate 2-hydroxymuconate. This proton transfer process is an essential part of degradative metabolism pathway to convert various aromatic hydrocarbons into their corresponding intermediates in the Krebs cycle Pseudomonas putida