EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.5 | expressed in Escherichia coli | Rattus norvegicus |
3.6.1.5 | recombinant expression of the mutant NTPDase1 ECD DELTAMIL in Escherichia coli in soluble form and refolding to the active state | Rattus norvegicus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.5 | NTPDase1 that lacks the two transmembrane helices and an additional putative membrane interaction loop, hanging drop vapor diffusion method, using 0.1 M sodium acetate (pH 4.5), 3.6 M sodium chloride | Rattus norvegicus |
3.6.1.5 | purified recombinant wild-type enzyme, free or in complex with decavanadate or heptamolybdate, X-ray diffraction structure determination and analysis at 1.7-2.5 A resolution, modeling | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.5 | K257M | site-directed mutagenesis | Rattus norvegicus |
3.6.1.5 | additional information | replacement of the extracellular domain of Rattus norvegicus NTPDase1, i.e. amino acid sequence 190TQEQSWLNFISDSQKQA206, with the shorter hydrophilic loop found in Homo sapiens NTPDase6, 240KTPGGS245. This NTPDase1 ECD DELTAMIL mutant variant is a soluble NTPDase1 that lacks a putative membrane interaction loop identified between the two lobes of the catalytic domain | Rattus norvegicus |
3.6.1.5 | Y409F | site-directed mutagenesis | Rattus norvegicus |
3.6.1.5 | Y413F | site-directed mutagenesis | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.5 | ammonium heptamolybdate | AHM, (NH4)6[Mo7O24] | Rattus norvegicus | |
3.6.1.5 | AMP | - |
Rattus norvegicus | |
3.6.1.5 | additional information | inhibition mechanism of polyoxometallates, overview | Rattus norvegicus | |
3.6.1.5 | polytungstate salt POM-1 | Na6[H2W12O40] | Rattus norvegicus | |
3.6.1.5 | Sodium vanadate | Na3VO4 | Rattus norvegicus | |
3.6.1.5 | UMP | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.5 | additional information | - |
additional information | kinetics, overview | Rattus norvegicus | |
3.6.1.5 | 0.0025 | - |
ADP | recombinant mutant EDC K257M, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0025 | - |
ADP | recombinant mutant EDC Y314F, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0047 | - |
ADP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0051 | - |
ADP | recombinant mutant EDC Y409F, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0059 | - |
ADP | recombinant mutant EDC DELTA MIL, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.009 | - |
GTP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.01 | - |
UTP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0105 | - |
IDP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0108 | - |
ITP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0113 | - |
UDP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0114 | - |
GDP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0129 | - |
UTP | recombinant mutant EDC DELTA MIL, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0131 | - |
GDP | in presence of 0.1 mM UMP, recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0175 | - |
UDP | recombinant mutant EDC DELTA MIL, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0488 | - |
GDP | in presence of 0.1 mM AMP, recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.149 | - |
GDP | in presence of 0.1 mM AHM, recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.1712 | - |
AMPCPP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.7495 | - |
TDP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.1.5 | extracellular | - |
Rattus norvegicus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.5 | Cl- | the active-site clefts of NTPDase1 contain three chloride ions, one of which is bound to the second phosphate-binding loop, apyrase conserved region 4, ACR4 | Rattus norvegicus | |
3.6.1.5 | additional information | complex structures with decavanadate and heptamolybdate show that both polyoxometallates bind electrostatically to a loop that is involved in binding of the nucleobase | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.5 | ATP + 2 H2O | Rattus norvegicus | - |
AMP + 2 phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.5 | Rattus norvegicus | P97687 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.6.1.5 | vascular system | CD39 and NTPDase1 are the dominant NTPDases | Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.5 | adenosine 5'-[(alpha,beta)-methyleno] triphosphate + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.6.1.5 | ADP + H2O | - |
Rattus norvegicus | AMP + phosphate | - |
? | |
3.6.1.5 | AMPCPP + H2O | low activity | Rattus norvegicus | ? | - |
? | |
3.6.1.5 | ATP + 2 H2O | - |
Rattus norvegicus | AMP + 2 phosphate | - |
? | |
3.6.1.5 | ATP + H2O | - |
Rattus norvegicus | ADP + phosphate | - |
? | |
3.6.1.5 | GDP + H2O | - |
Rattus norvegicus | GMP + phosphate | - |
? | |
3.6.1.5 | GTP + 2 H2O | - |
Rattus norvegicus | GMP + 2 phosphate | - |
? | |
3.6.1.5 | IDP + H2O | - |
Rattus norvegicus | IMP + phosphate | - |
? | |
3.6.1.5 | ITP + 2 H2O | - |
Rattus norvegicus | IMP + 2 phosphate | - |
? | |
3.6.1.5 | ITP + H2O | - |
Rattus norvegicus | IDP + phosphate | - |
? | |
3.6.1.5 | additional information | establishment of a kinetic isothermal titration calorimetry assay. Substrate recognition by NTPDase1, overview | Rattus norvegicus | ? | - |
? | |
3.6.1.5 | additional information | no hydrolysis of AMPCP | Rattus norvegicus | ? | - |
? | |
3.6.1.5 | TDP + H2O | - |
Rattus norvegicus | TMP + phosphate | - |
? | |
3.6.1.5 | TTP + 2 H2O | weak substrate | Rattus norvegicus | TMP + 2 phosphate | - |
? | |
3.6.1.5 | UDP + H2O | - |
Rattus norvegicus | UMP + phosphate | - |
? | |
3.6.1.5 | UTP + 2 H2O | - |
Rattus norvegicus | UMP + 2 phosphate | - |
? | |
3.6.1.5 | UTP + H2O | - |
Rattus norvegicus | UDP + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.5 | CD39 | - |
Rattus norvegicus |
3.6.1.5 | NTPDase 1 | - |
Rattus norvegicus |
3.6.1.5 | NTPDase1 | - |
Rattus norvegicus |
3.6.1.5 | nucleoside triphosphate diphosphohydrolase 1 | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.5 | 25 | - |
assay at | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.5 | 31.1 | - |
ADP | recombinant mutant EDC Y409F, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 40.5 | - |
AMPCPP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 50.4 | - |
ADP | recombinant mutant EDC K257M, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 77.3 | - |
ADP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 79.9 | - |
IDP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 80.2 | - |
TDP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 80.9 | - |
UDP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 80.9 | - |
GTP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 82.6 | - |
GDP | in presence of 0.1 mM AMP, recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 83.2 | - |
GDP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 83.2 | - |
GDP | in presence of 0.1 mM UMP, recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 86.1 | - |
ADP | recombinant mutant EDC Y314F, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 86.9 | - |
ADP | recombinant mutant EDC DELTA MIL, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 93 | - |
UDP | recombinant mutant EDC DELTA MIL, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 103.4 | - |
UTP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 103.7 | - |
GDP | in presence of 0.1 mM AHM, recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 105.3 | - |
ITP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 121.2 | - |
UTP | recombinant mutant EDC DELTA MIL, pH 7.4, 25°C | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.5 | 7.4 | - |
assay at | Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.5 | 0.00859 | - |
ammonium heptamolybdate | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0268 | - |
AMP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.0268 | - |
AMP | at pH 7.4 and 25°C | Rattus norvegicus | |
3.6.1.5 | 0.3345 | - |
UMP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 0.3345 | - |
UMP | at pH 7.4 and 25°C | Rattus norvegicus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.6.1.5 | evolution | the enzyme is a member of the eukaryotic NTPDase family | Rattus norvegicus |
3.6.1.5 | additional information | influence of transmembrane helix dynamics on activity is achieved by coupling to a domain motion. Active site structure of NTPDase1, overview, closure movement in NTPDases | Rattus norvegicus |
3.6.1.5 | physiological function | nucleoside triphosphate diphosphohydrolases are a physiologically important class of membrane-bound ectonucleotidases responsible for the regulation of extracellular levels of nucleotides | Rattus norvegicus |
3.6.1.5 | physiological function | the enzyme blocks platelet aggregation and supports blood flow | Rattus norvegicus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.5 | 110 | - |
TDP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 230 | - |
AMPCPP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 700 | - |
GDP | in presence of 0.1 mM AHM, recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 1700 | - |
GDP | in presence of 0.1 mM AMP, recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 5300 | - |
UDP | recombinant mutant EDC DELTA MIL, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 6100 | - |
ADP | recombinant mutant EDC Y409F, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 6400 | - |
GDP | in presence of 0.1 mM UMP, recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 7200 | - |
UDP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 7300 | - |
GDP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 7600 | - |
IDP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 9000 | - |
GTP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 9400 | - |
UTP | recombinant mutant EDC DELTA MIL, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 9800 | - |
ITP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 10300 | - |
UTP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 14700 | - |
ADP | recombinant mutant EDC DELTA MIL, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 16400 | - |
ADP | recombinant wild-type enzyme, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 20200 | - |
ADP | recombinant mutant EDC K257M, pH 7.4, 25°C | Rattus norvegicus | |
3.6.1.5 | 26900 | - |
ADP | recombinant mutant EDC Y314F, pH 7.4, 25°C | Rattus norvegicus |