BRENDA - Enzyme Database

Crystal structures and enzymatic properties of a triamine/agmatine aminopropyltransferase from Thermus thermophilus

Ohnuma, M.; Ganbe, T.; Terui, Y.; Niitsu, M.; Sato, T.; Tanaka, N.; Tamakoshi, M.; Samejima, K.; Kumasaka, T.; Oshima, T.; J. Mol. Biol. 408, 971-986 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.5.1.104
expression in Escherichia coli
Thermus thermophilus
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.5.1.104
hanging-drop vapor diffusion method
Thermus thermophilus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.00173
-
spermidine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
0.57
-
norspermidine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
0.77
-
agmatine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
1.64
-
S-adenosyl 3-(methylthio)propylamine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
38.31
-
tris(3-aminopropyl)amine
pH 9.0, 37C
Thermus thermophilus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.104
36006
-
4 * 36006, calculated from sequence
Thermus thermophilus
2.5.1.104
130000
-
gel filtration
Thermus thermophilus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.104
Thermus thermophilus
Q5SK28
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.5.1.104
-
Thermus thermophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.104
additional information
only faint activities (less than 10% of spermidine) are detected for diaminopropane [NH2(CH2)3NH2], putrescine, spermine, and caldopentamine
720250
Thermus thermophilus
?
-
-
-
-
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
-
720250
Thermus thermophilus
S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + homospermidine
low activity
720250
Thermus thermophilus
S-methyl-5'-thioadenosine + ?
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + N,N'-bis(3-aminopropyl)-1,3-propanediamine
i.e. thermine. Low activity
720250
Thermus thermophilus
S-methyl-5'-thioadenosine + ?
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + norspermidine
-
720250
Thermus thermophilus
S-methyl-5'-thioadenosine + N,N'-bis(3-aminopropyl)-1,3-propanediamine
i.e. thermine
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + spermidine
-
720250
Thermus thermophilus
S-methyl-5'-thioadenosine + spermine
no formation of thermospermine (i.e. 1,12-diamino-4,8-diazadodecane)
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + tris(3-aminopropyl)amine
i.e. mitsubishine. Low activity. No formation of tetrakis(3-aminopropyl)ammonium
720250
Thermus thermophilus
S-methyl-5'-thioadenosine + N4-aminopropylthermine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.5.1.104
homotetramer
4 * 36006, calculated from sequence
Thermus thermophilus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.5.1.104
37
-
assay at
Thermus thermophilus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.29
-
tris(3-aminopropyl)amine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
0.37
-
agmatine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
0.53
-
norspermidine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
0.65
-
spermidine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
0.73
-
S-adenosyl 3-(methylthio)propylamine
pH 9.0, 37C
Thermus thermophilus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.104
8.5
-
at 60C
Thermus thermophilus
2.5.1.104
9
-
assay at
Thermus thermophilus
2.5.1.104
9.5
-
at 37C
Thermus thermophilus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.5.1.104
10
-
stable around pH 10.0 at room temperature
Thermus thermophilus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.104
expression in Escherichia coli
Thermus thermophilus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.5.1.104
hanging-drop vapor diffusion method
Thermus thermophilus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.00173
-
spermidine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
0.57
-
norspermidine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
0.77
-
agmatine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
1.64
-
S-adenosyl 3-(methylthio)propylamine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
38.31
-
tris(3-aminopropyl)amine
pH 9.0, 37C
Thermus thermophilus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.104
36006
-
4 * 36006, calculated from sequence
Thermus thermophilus
2.5.1.104
130000
-
gel filtration
Thermus thermophilus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.104
-
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.104
additional information
only faint activities (less than 10% of spermidine) are detected for diaminopropane [NH2(CH2)3NH2], putrescine, spermine, and caldopentamine
720250
Thermus thermophilus
?
-
-
-
-
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + agmatine
-
720250
Thermus thermophilus
S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + homospermidine
low activity
720250
Thermus thermophilus
S-methyl-5'-thioadenosine + ?
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + N,N'-bis(3-aminopropyl)-1,3-propanediamine
i.e. thermine. Low activity
720250
Thermus thermophilus
S-methyl-5'-thioadenosine + ?
-
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + norspermidine
-
720250
Thermus thermophilus
S-methyl-5'-thioadenosine + N,N'-bis(3-aminopropyl)-1,3-propanediamine
i.e. thermine
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + spermidine
-
720250
Thermus thermophilus
S-methyl-5'-thioadenosine + spermine
no formation of thermospermine (i.e. 1,12-diamino-4,8-diazadodecane)
-
-
?
2.5.1.104
S-adenosyl 3-(methylthio)propylamine + tris(3-aminopropyl)amine
i.e. mitsubishine. Low activity. No formation of tetrakis(3-aminopropyl)ammonium
720250
Thermus thermophilus
S-methyl-5'-thioadenosine + N4-aminopropylthermine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.5.1.104
homotetramer
4 * 36006, calculated from sequence
Thermus thermophilus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.5.1.104
37
-
assay at
Thermus thermophilus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.104
0.29
-
tris(3-aminopropyl)amine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
0.37
-
agmatine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
0.53
-
norspermidine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
0.65
-
spermidine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
0.73
-
S-adenosyl 3-(methylthio)propylamine
pH 9.0, 37C
Thermus thermophilus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.104
8.5
-
at 60C
Thermus thermophilus
2.5.1.104
9
-
assay at
Thermus thermophilus
2.5.1.104
9.5
-
at 37C
Thermus thermophilus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.5.1.104
10
-
stable around pH 10.0 at room temperature
Thermus thermophilus
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.5.1.104
7.6
-
tris(3-aminopropyl)amine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
375
-
spermidine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
450
-
S-adenosyl 3-(methylthio)propylamine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
480
-
agmatine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
920
-
norspermidine
pH 9.0, 37C
Thermus thermophilus
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.5.1.104
7.6
-
tris(3-aminopropyl)amine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
375
-
spermidine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
450
-
S-adenosyl 3-(methylthio)propylamine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
480
-
agmatine
pH 9.0, 37C
Thermus thermophilus
2.5.1.104
920
-
norspermidine
pH 9.0, 37C
Thermus thermophilus