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Literature summary extracted from

  • Ralat, L.A.; Kalas, V.; Zheng, Z.; Goldman, R.D.; Sosnick, T.R.; Tang, W.J.
    Ubiquitin is a novel substrate for human insulin-degrading enzyme (2011), J. Mol. Biol., 406, 454-466.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.56 expressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.56 E341A mutant is active in degrading substrate V, relative activity closed to wild-type Homo sapiens
3.4.24.56 E341K mutant is active in degrading substrate V, relative activity 15% compared to wild-type. Exosite mutant is unable to further degrade the Ub1-74 fragment Homo sapiens
3.4.24.56 E341Q mutant is active in degrading substrate V, relative activity 20% compared to wild-type. Exosite mutant is unable to further degrade the Ub1-74 fragment Homo sapiens
3.4.24.56 G339P mutant is active in degrading substrate V, relative activity 75% compared to wild-type. Exosite mutant is unable to further degrade the Ub1-74 fragment Homo sapiens
3.4.24.56 G361P mutant is active in degrading substrate V, relative activity 80% compared to wild-type. Exosite mutant is unable to further degrade the Ub1-74 fragment Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.56 nestin potently inhibits the cleavage of ubiquitin by IDE Homo sapiens
3.4.24.56 Ub1-72 cleaved ubiquitin Homo sapiens
3.4.24.56 Ub1-74 cleaved ubiquitin Homo sapiens
3.4.24.56 ubiquitin
-
Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.56 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.56 using Ni-NTA chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.56 (7-methoxycoumarin-4-yl)acetyl-NPPGFSAFK-2,4-dinitrophenyl + H2O bradykinin mimetic substrate V Homo sapiens ?
-
?
3.4.24.56 ubiquitin + H2O IDE cleaves ubiquitin in a biphasic manner, first, by rapidly removing the two C-terminal glycines (kcat = 2/sec) followed by a slow cleavage between residues 72-73 (kcat = 0.07/sec), thereby producing the inactive Ub1-74 and Ub1-72 Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.56 IDE
-
Homo sapiens
3.4.24.56 Insulysin
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.56 37
-
assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.56 0.028
-
(7-methoxycoumarin-4-yl)acetyl-NPPGFSAFK-2,4-dinitrophenyl pH not specified in the publication, 37°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.24.56 0.001
-
pH not specified in the publication, 37°C Homo sapiens Ub1-72
3.4.24.56 0.09
-
pH not specified in the publication, 37°C Homo sapiens ubiquitin
3.4.24.56 0.1
-
pH not specified in the publication, 37°C Homo sapiens Ub1-72