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Literature summary extracted from

  • Kerff, F.; Petrella, S.; Mercier, F.; Sauvage, E.; Herman, R.; Pennartz, A.; Zervosen, A.; Luxen, A.; Fr่re, J.; Joris, B.; Charlier, P.
    Specific structural features of the N-acetylmuramoyl-L-alanine amidase amid from Escherichia coli and mechanistic implications for enzymes of this family (2010), J. Mol. Biol., 397, 249-259.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.28 expressed in Escherichia coli LMG194 and B180 cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.28 hanging drop vapor diffusion method, using 1 M LiCl, 10% (w/v) polyethylene glycol 6000 in a 0.1 M sodium citrate buffer (pH 4) for the native structure, and 1 M MgSO4 in a 0.1 M sodium acetate buffer (pH 4.6) for the Se-Met structure. The apoenzyme in complex with the substrate anhydro-N-acetylmuramic-acid-L-Ala-gamma-D-Glu-L-Lys is crystallized using 50% (w/v) polyethylene glycol 6000, 0.1 M sodium citrate buffer (pH 4), and 5 mM EDTA. The holoenzyme in complex with the product L-Ala-gamma-D-Glu-L-Lys is crystallized using 1 M MgCl2 in a 0.1 M sodium acetate buffer, pH 4.6 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.28 periplasm
-
 Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.28 Zn2+ contains zinc Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.28 Escherichia coli P75820
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.28 S-Sepharose column chromatography, Q-Sepharose column chromatography, and Sephacryl S100 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.28 1,6-anhydro-N-acetylmuramic-acid-L-Ala-gamma-D-Glu-L-Lys + H2O
-
 Escherichia coli 1,6-anhydro-N-acetylmuramate + L-Ala-gamma-D-Glu-L-Lys
-
 ?
3.5.1.28 additional information the enzyme has no activity on MurNAc-L-Ala-D-Glu Escherichia coli ?
-
 ?
3.5.1.28 N-acetylmuramoyl-L-alanine + H2O
-
 Escherichia coli N-acetylmuramate + L-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.28 homodimer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.5.1.28 AmiD
-
 Escherichia coli