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Literature summary extracted from

  • Lee, Y.G.; Leem, S.H.; Chung, Y.H.; Kim, S.I.
    Characterization of thermostable deblocking aminopeptidases of archaeon Thermococcus onnurineus NA1 by proteomic and biochemical approaches (2012), J. Microbiol., 50, 792-797.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.B5 expression in Escherichia coli Thermococcus onnurineus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.B5 Zn2+
-
 Thermococcus onnurineus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.B5 0.46
-
 L-methionyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus
3.4.11.B5 2.65
-
 L-leucyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus
3.4.11.B5 10.9
-
 L-methionyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus
3.4.11.B5 37.5
-
 L-leucyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.B5 Ba2+ activates Thermococcus onnurineus
3.4.11.B5 Ba2+ activates, relative activity is about 90% compared to Mn2+ Thermococcus onnurineus
3.4.11.B5 Co2+ activates, 0.1 mM Co2+ in assay mixture Thermococcus onnurineus
3.4.11.B5 Co2+ activates, relative activity is about 80% compared to Mn2+, 0.1 mM Co2+ in assay mixture Thermococcus onnurineus
3.4.11.B5 Cu2+ relative activity is about 30% compared to Mn2+ Thermococcus onnurineus
3.4.11.B5 Cu2+ relative activity is about 35% compared to Mg2+ Thermococcus onnurineus
3.4.11.B5 Mg2+ activates Thermococcus onnurineus
3.4.11.B5 Mg2+ activates, relative activity is about 80% compared to Mn2+ Thermococcus onnurineus
3.4.11.B5 Mn2+ activates Thermococcus onnurineus
3.4.11.B5 Mn2+ relative activity is about 75% compared to Mg2+ Thermococcus onnurineus
3.4.11.B5 additional information metal binding residues are His71, Asp188, Asp 244, and Glu222 Thermococcus onnurineus
3.4.11.B5 Ni2+ relative activity is about 40% compared to Mn2+ Thermococcus onnurineus
3.4.11.B5 Ni2+ relative activity is about 80% compared to Mg2+ Thermococcus onnurineus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.B5 36470
-
 4 * 36470, calculated from sequence Thermococcus onnurineus
3.4.11.B5 37360
-
 2 * 37360, calculated from sequence Thermococcus onnurineus
3.4.11.B5 37950
-
 14 * 37950, calculated from sequence Thermococcus onnurineus
3.4.11.B5 76100
-
 MALDI-TOF mas spectrometry Thermococcus onnurineus
3.4.11.B5 148000
-
 isoenzyme TNA1-DAP3, MALDI-TOF mass spectrometry Thermococcus onnurineus
3.4.11.B5 539600
-
 MALDI-TOF mass spectrometry Thermococcus onnurineus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.B5 Thermococcus onnurineus
-
-
-
3.4.11.B5 Thermococcus onnurineus A7J1X4
-
-
3.4.11.B5 Thermococcus onnurineus B6YTG7
-
-
3.4.11.B5 Thermococcus onnurineus B6YUS8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.B5
-
 Thermococcus onnurineus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.B5 L-leucyl 4-nitroanilide + H2O
-
 Thermococcus onnurineus L-leucine + 4-nitroaniline
-
 ?
3.4.11.B5 L-methionyl 4-nitroanilide + H2O
-
 Thermococcus onnurineus L-methionine + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.B5 dimer 2 * 37360, calculated from sequence Thermococcus onnurineus
3.4.11.B5 tetradecamer 14 * 37950, calculated from sequence Thermococcus onnurineus
3.4.11.B5 tetramer 4 * 36470, calculated from sequence Thermococcus onnurineus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.B5 TNA1-DAP1
-
 Thermococcus onnurineus
3.4.11.B5 TNA1-DAP2
-
 Thermococcus onnurineus
3.4.11.B5 TNA1-DAP3
-
 Thermococcus onnurineus
3.4.11.B5 TNA1-DAP4
-
 Thermococcus onnurineus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.B5 80
-
 assay at Thermococcus onnurineus
3.4.11.B5 90
-
-
 Thermococcus onnurineus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.B5 60 100 60°C: about 60% of maximal activity, 80°C: about 90% of maximal activity, 100°C: about 95% of maximal activity Thermococcus onnurineus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.B5 96
-
 half-life: about 2 h Thermococcus onnurineus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.B5 101
-
 L-methionyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus
3.4.11.B5 108
-
 L-leucyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.B5 7.5
-
 assay at Thermococcus onnurineus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.B5 41
-
 L-leucyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus
3.4.11.B5 220
-
 L-methionyl 4-nitroanilide pH 7.5, 80°C Thermococcus onnurineus