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Literature summary extracted from
- Molecular characterization of bile salt hydrolase from Bifidobacterium animalis subsp. lactis Bi30 (2011), J. Microbiol. Biotechnol., 21, 838-845.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.24 | MgCl2 | 107.6% activity at 30 mM | Bifidobacterium animalis subsp. lactis |  |
| 3.5.1.24 | MgSO4 | 112.6% activity at 30 mM | Bifidobacterium animalis subsp. lactis | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.24 | expressed in Escherichia coli XL1 Blue cells | Bifidobacterium animalis subsp. lactis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.24 | Ca2+ | 91.2% residual activity at 30 mM | Bifidobacterium animalis subsp. lactis | |
| 3.5.1.24 | Cu2+ | 1.4% residual activity at 5 mM | Bifidobacterium animalis subsp. lactis | |
| 3.5.1.24 | EDTA | 75.7% residual activity at 30 mM | Bifidobacterium animalis subsp. lactis | |
| 3.5.1.24 | Hg2+ | 2.2% residual activity at 0.2 mM | Bifidobacterium animalis subsp. lactis | |
| 3.5.1.24 | iodoacetamide | 75.8% residual activity at 30 mM | Bifidobacterium animalis subsp. lactis | |
| 3.5.1.24 | periodic acid | 0.6% residual activity at 3 mM | Bifidobacterium animalis subsp. lactis | |
| 3.5.1.24 | phenylmethylsulfonyl fluoride | 90.9% residual activity at 0.5 mM | Bifidobacterium animalis subsp. lactis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 35000 | - |
4 * 35000, SDS-PAGE | Bifidobacterium animalis subsp. lactis |
| 3.5.1.24 | 120000 | 140000 | gel filtration | Bifidobacterium animalis subsp. lactis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.24 | glycochenodeoxycholic acid + H2O | Bifidobacterium animalis subsp. lactis | about 75% activity compared to glycocholic acid | chenodeoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycochenodeoxycholic acid + H2O | Bifidobacterium animalis subsp. lactis Bi30 | about 75% activity compared to glycocholic acid | chenodeoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycocholic acid + H2O | Bifidobacterium animalis subsp. lactis | 100% activity | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine | - |
? | |
| 3.5.1.24 | glycocholic acid + H2O | Bifidobacterium animalis subsp. lactis Bi30 | 100% activity | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine | - |
? | |
| 3.5.1.24 | glycodeoxycholic acid + H2O | Bifidobacterium animalis subsp. lactis | about 80% activity compared to glycocholic acid | deoxycholate + glycine | - |
? | |
| 3.5.1.24 | additional information | Bifidobacterium animalis subsp. lactis | the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms | ? | - |
? | |
| 3.5.1.24 | additional information | Bifidobacterium animalis subsp. lactis Bi30 | the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms | ? | - |
? | |
| 3.5.1.24 | taurochenodeoxycholic acid + H2O | Bifidobacterium animalis subsp. lactis | about 15% activity compared to glycocholic acid | chenodeoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurochenodeoxycholic acid + H2O | Bifidobacterium animalis subsp. lactis Bi30 | about 15% activity compared to glycocholic acid | chenodeoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurocholic acid + H2O | Bifidobacterium animalis subsp. lactis | about 20% activity compared to glycocholic acid | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine | - |
? | |
| 3.5.1.24 | taurocholic acid + H2O | Bifidobacterium animalis subsp. lactis Bi30 | about 20% activity compared to glycocholic acid | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine | - |
? | |
| 3.5.1.24 | taurodeoxycholic acid + H2O | Bifidobacterium animalis subsp. lactis | less than 20% activity compared to glycocholic acid | deoxycholate + taurine | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.24 | Bifidobacterium animalis subsp. lactis | G0YYC2 | - |
- |
| 3.5.1.24 | Bifidobacterium animalis subsp. lactis Bi30 | G0YYC2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.24 | Q-Sepharose column chromatography and Sephacryl S-300 gel filtration | Bifidobacterium animalis subsp. lactis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.24 | glycochenodeoxycholic acid + H2O | about 75% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis | chenodeoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycochenodeoxycholic acid + H2O | about 75% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis Bi30 | chenodeoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycocholic acid + H2O | 100% activity | Bifidobacterium animalis subsp. lactis | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine | - |
? | |
| 3.5.1.24 | glycocholic acid + H2O | 100% activity | Bifidobacterium animalis subsp. lactis Bi30 | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine | - |
? | |
| 3.5.1.24 | glycodeoxycholic acid + H2O | about 80% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis | deoxycholate + glycine | - |
? | |
| 3.5.1.24 | additional information | the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms | Bifidobacterium animalis subsp. lactis | ? | - |
? | |
| 3.5.1.24 | additional information | the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms | Bifidobacterium animalis subsp. lactis Bi30 | ? | - |
? | |
| 3.5.1.24 | taurochenodeoxycholic acid + H2O | about 15% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis | chenodeoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurochenodeoxycholic acid + H2O | about 15% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis Bi30 | chenodeoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurocholic acid + H2O | about 20% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine | - |
? | |
| 3.5.1.24 | taurocholic acid + H2O | about 20% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis Bi30 | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine | - |
? | |
| 3.5.1.24 | taurodeoxycholic acid + H2O | less than 20% activity compared to glycocholic acid | Bifidobacterium animalis subsp. lactis | deoxycholate + taurine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.24 | homotetramer | 4 * 35000, SDS-PAGE | Bifidobacterium animalis subsp. lactis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.24 | bile salt hydrolase | - |
Bifidobacterium animalis subsp. lactis |
| 3.5.1.24 | BSH | - |
Bifidobacterium animalis subsp. lactis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 50 | - |
around 50°C | Bifidobacterium animalis subsp. lactis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 45 | 55 | 85% and 82% of maximum activity at 45°C and 55°C, respectively | Bifidobacterium animalis subsp. lactis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 60 | 70 | a significant decrease in enzyme activity is observed at temperatures higher than 60°C. At 70°C, 90% of the catalytic activity is lost | Bifidobacterium animalis subsp. lactis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 5.1 | - |
- |
Bifidobacterium animalis subsp. lactis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 4.7 | 6.5 | about 50% activity at pH 4.7 and 6.5 | Bifidobacterium animalis subsp. lactis |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.5.1.24 | Bifidobacterium animalis subsp. lactis | isoelectric focusing | - |
4.9 |
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