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Literature summary extracted from

  • Kim, B.; Kim, I.; Ahn, J.
    Characterization of hydroxycinnamoyl-coenzyme A shikimate hydroxycinnamoyltransferase from Populus euramericana (2011), J. Korean Soc. Appl. Biol. Chem., 54, 817-821.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.133 expression of GST-tagged HCT in Escherichia coli strain BL21 (DE3) Populus x canadensis
2.3.1.133 genes PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7, expression as GST-tagged enzymes in Escherichia coli strain BL21 (DE3) Populus x canadensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.133 additional information
-
 additional information kinetics, overview Populus x canadensis
2.3.1.133 0.0009
-
 shikimate pH 8.0, 37°C, recombinant GST-tagged enzyme, with 4-coumaroyl-CoA Populus x canadensis
2.3.1.133 0.00582
-
 quinate pH 8.0, 37°C, recombinant GST-tagged enzyme, with 4-coumaroyl-CoA Populus x canadensis
2.3.1.133 0.00813
-
 4-coumaroyl-CoA pH 8.0, 37°C, recombinant GST-tagged enzyme, with shikimate Populus x canadensis
2.3.1.133 0.00964
-
 4-coumaroyl-CoA pH 8.0, 37°C, recombinant GST-tagged enzyme, with quinate Populus x canadensis
2.3.1.133 0.9
-
 shikimate with 4-coumaroyl-CoA, pH 8.0, 37°C Populus x canadensis
2.3.1.133 5.82
-
 quinate with 4-coumaroyl-CoA, pH 8.0, 37°C Populus x canadensis
2.3.1.133 8.13
-
 4-coumaroyl-CoA with shikimate, pH 8.0, 37°C Populus x canadensis
2.3.1.133 9.64
-
 4-coumaroyl-CoA with quinate, pH 8.0, 37°C Populus x canadensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.133 70000
-
 x * 70000, about, recombinant GST-tagged HCT, SDS-PAGE Populus x canadensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.133 4-coumaroyl-CoA + quinate Populus x canadensis
-
 CoA + 5-(4-coumaroyl)quinate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate Populus x canadensis recombinant HCT shows substrate-specificity towards shikimic acid in contrast to hydroxycinnamoyl-CoA quinate transferase, HQT, EC 2.3.1.99 CoA + 4-coumaroylshikimate
-
 ?
2.3.1.133 additional information Populus x canadensis HCT mediates formation of hydroxycinnamoylquinnate or hydroxycinnamoylshikimate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.133 Populus x canadensis
-
-
-
2.3.1.133 Populus x canadensis
-
 genes PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.133 recombinant GST-tagged HCT from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography Populus x canadensis
2.3.1.133 recombinant GST-tagged PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7 gene products from Escherichia coli strain BL21 (DE3) by glutathione affinity cromatography Populus x canadensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.133 leaf
-
 Populus x canadensis
-
2.3.1.133 additional information PeHCT displays 1.5fold higher expression in the stem than in the leaf, and its expression increases during the growth stage of the leaf Populus x canadensis
-
2.3.1.133 petiole
-
 Populus x canadensis
-
2.3.1.133 stem
-
 Populus x canadensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.133 4-coumaroyl-CoA + quinate preferred substrate is shikimate Populus x canadensis CoA + 4-coumaroylquinate
-
 ?
2.3.1.133 4-coumaroyl-CoA + quinate
-
 Populus x canadensis CoA + 5-(4-coumaroyl)quinate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate recombinant HCT shows substrate-specificity towards shikimic acid in contrast to hydroxycinnamoyl-CoA quinate transferase, HQT, EC 2.3.1.99 Populus x canadensis CoA + 4-coumaroylshikimate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate preferred substrate is shikimate Populus x canadensis CoA + 4-coumaroylshikimate
-
 ?
2.3.1.133 additional information HCT mediates formation of hydroxycinnamoylquinnate or hydroxycinnamoylshikimate Populus x canadensis ?
-
 ?
2.3.1.133 additional information in vitro recombinant PeHCT shows substrate-specificity towards shikimic acid Populus x canadensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.133 ? x * 70000, about, recombinant GST-tagged HCT, SDS-PAGE Populus x canadensis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.133 HCT
-
 Populus x canadensis
2.3.1.133 hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase
-
 Populus x canadensis
2.3.1.133 shikimate hydroxycinnamoyltransferase
-
 Populus x canadensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.133 37
-
 assay at Populus x canadensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.133 8
-
 assay at Populus x canadensis

General Information

EC Number General Information Comment Organism
2.3.1.133 evolution HCT is a member of the BAHD [BEAT (benzylalcohol O-acetyltransferase)] family acyltransferases Populus x canadensis
2.3.1.133 evolution HCT is a member of the BAHD, i.e. BEAT (benzylalcohol O-acetyltransferase), family acyltransferases Populus x canadensis
2.3.1.133 metabolism HCT is likely to be involved in lignin biosynthesis, overview Populus x canadensis
2.3.1.133 metabolism PeHCT might be involved in lignin biosynthesis by formation of 4-coumaroyl shikimate Populus x canadensis
2.3.1.133 additional information PeHCT contains a conserved HXXXDG motif that is found in acyltransferase Populus x canadensis