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Literature summary extracted from

  • Vidal, R.F.; Martinez, A.; Moulis, C.; Escalier, P.; Morel, S.; Remaud-Simeon, M.; Monsan, P.
    A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives (2011), J. Ind. Microbiol. Biotechnol., 38, 1499-1506.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.5 synthesis the strain Leuconostoc citreum strain B/110-1-2 is used for industrial production of dextran and dextran derivatives Leuconostoc citreum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.5 two ORFs coding for strain B/110-1-2 dextransucrases, DNA and amino acid sequence determination and analysis, sequence comparisons Leuconostoc citreum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.5 extracellular
-
Leuconostoc citreum
-
-
2.4.1.5 intracellular
-
Leuconostoc citreum 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 160000 180000 intracellular dextransucrase 1 and extracellular dextransucrase Leuconostoc citreum
2.4.1.5 220000 240000 intracellular dextransucrse 2 Leuconostoc citreum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.5 additional information Leuconostoc citreum structural analysis, by HPAEC-PAD, HPSEC, and 13C-NMR, of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases, overview ?
-
?
2.4.1.5 additional information Leuconostoc citreum B/110-1-2 structural analysis, by HPAEC-PAD, HPSEC, and 13C-NMR, of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases, overview ?
-
?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc citreum
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc citreum B/110-1-2
-
D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc citreum D5FS20 isolated from sugarcane juice
-
2.4.1.5 Leuconostoc citreum B/110-1-2 D5FS20 isolated from sugarcane juice
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 additional information structural analysis, by HPAEC-PAD, HPSEC, and 13C-NMR, of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases, overview Leuconostoc citreum ?
-
?
2.4.1.5 additional information structural analysis, by HPAEC-PAD, HPSEC, and 13C-NMR, of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases, overview Leuconostoc citreum B/110-1-2 ?
-
?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc citreum D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n
-
Leuconostoc citreum B/110-1-2 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 30
-
assay at Leuconostoc citreum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.4
-
assay at Leuconostoc citreum

General Information

EC Number General Information Comment Organism
2.4.1.5 physiological function in presence of sucrose, the strain B/110-1-2 produces two cell-associated dextransucrases (31.54% of the total glucosyltransferase activity) with molecular weights of 160 and 240 kDa and a soluble dextransucrase (68.46% of the total glucosyltransferase activity) at 160-180 kDa Leuconostoc citreum