Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nakao, H.; Shinoda, S.; Yamamoto, S.
    Purification and properties of carboxynorspermidine decarboxylase, a novel enzyme involved in norspermidine biosynthesis, from Vibrio alginolyticus (1990), J. Gen. Microbiol., 136, 1699-1704.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.96 dithiothreitol maximum stimulation of activity at more than 5 mM dithiothreitol Vibrio alginolyticus

General Stability

EC Number General Stability Organism
4.1.1.96 freezing and thawing the purified preparation results in almost complete loss of activity. A substantial loss of activity (about 20%) is observed when the enzyme is dialysed for 12 h or stored at 4°C for 2 days without addition of EDTA Vibrio alginolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.96 Fe2+ 82% inhibition at 1 mM Vibrio alginolyticus
4.1.1.96 Fe3+ 88% inhibition at 1 mM Vibrio alginolyticus
4.1.1.96 Mn2+ 50% inhibition at 1 mM Vibrio alginolyticus
4.1.1.96 additional information not inhibited by 2,3-diaminopropionic acid, 2,4-diaminobutyric acid, L-ornithine, L-lysine, Mg2+, Ca2+, Cu2+, Cu+, Na+ and K+ Vibrio alginolyticus
4.1.1.96 Zn2+ 23% inhibition at 1 mM Vibrio alginolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.96 0.175
-
carboxynorspermidine at pH 8.3 and 37°C Vibrio alginolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.96 43500
-
2 * 43500, SDS-PAGE Vibrio alginolyticus
4.1.1.96 86000
-
gel filtration Vibrio alginolyticus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.96 Vibrio alginolyticus
-
-
-
4.1.1.96 Vibrio alginolyticus ATCC 17749
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.96 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Sephacryl S-200 gel filtration, and hydroxyapatite column chromatography. The enzyme is greatly stabilized by the addition of both dithiothreitol and EDTA, but not pyridoxal 5'-phosphate, during purification Vibrio alginolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.96 0.0077
-
crude extract, at pH 8.3 and 37°C Vibrio alginolyticus
4.1.1.96 24.1
-
after 3130fold purification, at pH 8.3 and 37°C Vibrio alginolyticus

Storage Stability

EC Number Storage Stability Organism
4.1.1.96 4°C, at pH 7.5 in the presence of dithiothreitol, EDTA and NaN, at least one month, the enzyme remains stable Vibrio alginolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.96 carboxynorspermidine
-
Vibrio alginolyticus bis(3-aminopropyl)amine + CO2
-
?
4.1.1.96 carboxynorspermidine
-
Vibrio alginolyticus ATCC 17749 bis(3-aminopropyl)amine + CO2
-
?
4.1.1.96 carboxyspermidine alternative substrate, is decarboxylated at almost the same rate as carboxynorspermidine Vibrio alginolyticus spermidine + CO2
-
?
4.1.1.96 carboxyspermidine alternative substrate, is decarboxylated at almost the same rate as carboxynorspermidine Vibrio alginolyticus ATCC 17749 spermidine + CO2
-
?
4.1.1.96 additional information neither 2,3-diaminopropionic acid, 2,4-diaminobutyric acid, L-ornithine nor L-lysine show detectable substrate activity Vibrio alginolyticus ?
-
?
4.1.1.96 additional information neither 2,3-diaminopropionic acid, 2,4-diaminobutyric acid, L-ornithine nor L-lysine show detectable substrate activity Vibrio alginolyticus ATCC 17749 ?
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.96 homodimer 2 * 43500, SDS-PAGE Vibrio alginolyticus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.96 C-NSPD decarboxylase
-
Vibrio alginolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.96 37
-
-
Vibrio alginolyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.96 20 55 75% and 51% of the maximum activity remains at 30°C and 45°C, respectively, but the activity decreases rapidly at temperatures above 55°C or below 20°C Vibrio alginolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.96 8.3
-
-
Vibrio alginolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.96 7.5 9 57% and 72% of the maximum activity are observed at pH 7.5 and 9.0, respectively Vibrio alginolyticus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.96 pyridoxal 5'-phosphate Km value is 0.0048 mM Vibrio alginolyticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.96 Vibrio alginolyticus isoelectric focusing
-
4.3

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.1.96 1
-
at pH 8.3 and 37°C Vibrio alginolyticus Mn2+